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Research Article

美他沙酮抑制小鼠巨噬细胞RAW264.7细胞中与疼痛相关的炎性细胞因子的产生:与β-石竹烯协同作用

卷 20, 期 8, 2020

页: [643 - 652] 页: 10

弟呕挨: 10.2174/1566524020666200217102508

价格: $65

摘要

背景和目的：炎症与许多疾病的发病机制有关。伴有疼痛的炎症细胞因子是众所周知的人类肌肉疼痛的生物标记物，它们由巨噬细胞产生。美他沙酮被用作骨骼肌松弛剂，但其作用机制尚不清楚。本研究旨在探讨美他沙酮在体外是否对炎性巨噬细胞活性有抑制作用。 方法：小鼠巨噬细胞RAW264.7细胞在含有10%胎牛血清的杜尔贝科修饰的鹰培养液中培养。计数附在培养皿上的细胞数，检测细胞生长情况。用ELISA试剂盒分析释放到培养基中的炎性细胞因子。 结果：在体外实验中发现美他沙酮(1-100μM)通过抑制RAW264.7细胞的增殖和刺激细胞死亡来减少巨噬细胞的数量。美他沙酮(0.1或1μM)和β-石竹烯(10或50μM)单独作用对细胞数量没有显著影响，但对RAW264.7细胞的生长和死亡有潜在的影响。机械地说，用美他沙酮或β-石竹烯治疗可以降低丝裂原活化蛋白激酶的分子水平，并且两者联合使用可以增强每种效果。此外，美他沙酮或β-石竹烯可增加caspase-3的水平，并通过二者的联合增强。值得注意的是,制作的炎性细胞因子,包括肿瘤坏死因子-

关键词: β-石柱烯，炎性细胞因子，COX-1

Erratum For:
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