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Current Molecular Medicine

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ISSN (Print): 1566-5240
ISSN (Online): 1875-5666

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Research Article

An In vivo Immunohistochemical Study on MacroH2A.1 in Lung and Lymph-Node Tissues Exposed to an Asbestiform Fiber

Author(s): Carla Loreto*, Claudia Lombardo, Rosario Caltabiano, Caterina Ledda, Maria Hagnas, Vera Filetti and Venerando Rapisarda

Volume 20, Issue 8, 2020

Page: [653 - 660] Pages: 8

DOI: 10.2174/1566524020666200220130023

Price: $65

Abstract

Aims: The aim of this study was to investigate MacroH2A.1 immunoexpression in tissues of sheep exposed to FE.

Background: The correlation between asbestiform fibers, lung cancer, pleural mesothelioma, and other lung diseases is already well established as the pathophisiological pathophysiological respiratory mechanisms involved by inhalation of Fluoro-edenite (FE). The latter is represented by cell proliferation and inducing the release of growth factors, cytokines, and reactive oxygen and nitrite species, with DNA damage that causes chronic inflammation and carcinogenesis. MacroH2A.1, and histone variant, seems to play a role in sensing the metabolic state of the cell and linking it with chromatin. Physiologically, MacroH2A.1 is expressed at low levels in stem cells and it became upregulated during differentiation, preventing reprogramming of induced pluripotent stem cells and after nuclear transfer. In particular, MacroH2A.1 has been shown to explicate a potent antitumor mechanism in vivo as it results upregulated in senescent cells determining a permanent growth-arrest.

Objective: Evaluate the possible role of the histone variant in the organism in response to deep insight understanding the mechanisms of toxicity and the cellular response to FE.

Methods: Lung and lymph nodes of exposed sheep were selected. Samples were processed for histological and immunihistochemical immunohistochemical evaluations. Densitometric, morphometric, and statistical analysis analyses were conducted.

Results: Tissue sections of FE exposed sheep demonstrated overexpression of MacroH2A.1 vs unexposed samples. The data suggest an involvement of these this molecule in the cellular response triggered by FE directed exposure.

Conclusion: In this contest, MacroH2A.1 overexpression supports its function as an epigenetic stabilizer that helps to establish and maintain differentiated states.

Keywords: Lung, Asbestos, Cancer, Tumor, H2AFY, Core histone Macro-H2A.1, Metabolism.

« Previous Next »
[1]
Gianfagna A, Oberti R. Fluoro-edenite from Biancavilla (Catania, Sicily, Italy): crystal chemistry of a new amphibole end-member. Am Mineral 2001; 1(86): 1489-93.
[2]
Cardile V, Lombardo L, Belluso E, et al. Fluoro-edenite fibers induce expression of Hsp70 and inflammatory response. Int J Environ Res Public Health 2007; 4(3): 195-202.
[http://dx.doi.org/10.3390/ijerph2007030001] [PMID: 17911657]
[3]
Di Paola M, Mastrantonio M, Carboni M. Mortality from malignant pleural neoplasms in Italy in the years 1988-1992. In: Rapporti ISTISAN 96/40. Rome. Italy. 1996.
[4]
Paoletti L, Batisti D, Bruno C, et al. Unusually high incidence of malignant pleural mesothelioma in a town of eastern Sicily: an epidemiological and environmental study. Arch Environ Health 2000; 55(6): 392-8.
[http://dx.doi.org/10.1080/00039890009604036] [PMID: 11128876]
[5]
Comba P, Gianfagna A, Paoletti L. Pleural mesothelioma cases in Biancavilla are related to a new fluoro-edenite fibrous amphibole. Arch Environ Health 2003; 58(4): 229-32.
[http://dx.doi.org/10.3200/AEOH.58.4.229-232] [PMID: 14655903]
[6]
Biggeri A, Pasetto R, Belli S, et al. Mortality from chronic obstructive pulmonary disease and pleural mesothelioma in an area contaminated by natural fiber (fluoro-edenite). Scand J Work Environ Health 2004; 30(3): 249-52.
[http://dx.doi.org/10.5271/sjweh.786] [PMID: 15250654]
[7]
Musumeci G, Cardile V, Fenga C, Caggia S, Loreto C. Mineral fibre toxicity: expression of retinoblastoma (Rb) and phospho-retinoblastoma (pRb) protein in alveolar epithelial and mesothelial cell lines exposed to fluoro-edenite fibres. Cell Biol Toxicol 2011; 27(3): 217-25.
[http://dx.doi.org/10.1007/s10565-011-9183-9] [PMID: 21327865]
[8]
Kamp DW, Weitzman SA. The molecular basis of asbestos induced lung injury. Thorax 1999; 54(7): 638-52.
[http://dx.doi.org/10.1136/thx.54.7.638] [PMID: 10377212]
[9]
Szychlinska MA, Parenti R, Loreto C, et al. Fluoro Edenite-associated pathogenesis in Pleural Malignant Mesothelioma. Acta Med Mediter 2014; 1(30): 981-8.
[10]
Rinaudo C, Cairo S, Gastaldi D, et al. Characterization of fluoro-edenite by m-Raman and m-FTIR spectroscopy. Mineral Mag 2006; 1(70): 291-8.
[http://dx.doi.org/10.1180/0026461067030332]
[11]
Martinez G, Loreto C, Rapisarda V, Masumeci G, Valentino M, Carnazza ML. Effects of exposure to fluoro-edenite fibre pollution on the respiratory system: an in vivo model. Histol Histopathol 2006; 21(6): 595-601.
[PMID: 16528669]
[12]
Loreto C, Carnazza ML, Cardile V, et al. Mineral fiber-mediated activation of phosphoinositide-specific phospholipase c in human bronchoalveolar carcinoma-derived alveolar epithelial A549 cells. Int J Oncol 2009; 34(2): 371-6.
[PMID: 19148471]
[13]
Loreto C, Rapisarda V, Carnazza ML, et al. Fluoro-edenite fibres induce lung cell apoptosis: an in vivo study. Histol Histopathol 2008; 23(3): 319-26.
[PMID: 18072089]
[14]
Musumeci G, Loreto C, Cardile V, Carnazza ML, Martinez G. Immunohistochemical expression of retinoblastoma and phospho-retinoblastoma protein in sheep lung exposed to fluoro-edenite fibers. Anat Sci Int 2010; 85(2): 74-8.
[http://dx.doi.org/10.1007/s12565-009-0059-5] [PMID: 19680741]
[15]
Musumeci G, Loreto C, Szychlinska MA, et al. N-Cadherin, ADAM-10 and Aquaporin 1 expression in lung tissue exposed to fluoro-edenite fibers: an immunohistochemical study. Histol Histopathol 2015; 30(8): 987-99.
[PMID: 25757887]
[16]
Caltabiano R, Loreto C, Vitale E, et al. Fibulin-3 immunoexpression in malignant mesothelioma due to fluoro-edenite: a preliminary report. Future Oncol 2018; 14(6s): 53-7.
[http://dx.doi.org/10.2217/fon-2017-0386] [PMID: 29664355]
[17]
Angelico G, Caltabiano R, Loreto C, et al. Immunohistochemical Expression of Aquaporin-1 in Fluoro-Edenite-Induced Malignant Mesothelioma: A Preliminary Report. Int J Mol Sci 2018; 19(3)E685
[http://dx.doi.org/10.3390/ijms19030685] [PMID: 29495596]
[18]
Rapisarda V, Caltabiano R, Musumeci G, et al. Analysis of fibulin-3 after exposure to asbestos-like fibers. Environ Res 2017; 156(156): 381-7.
[http://dx.doi.org/10.1016/j.envres.2017.03.055] [PMID: 28395242]
[19]
Musumeci G, Loreto C, Giunta S, et al. Angiogenesis correlates with macrophage and mast cell infiltration in lung tissue of animals exposed to fluoro-edenite fibers. Exp Cell Res 2016; 346(1): 91-8.
[http://dx.doi.org/10.1016/j.yexcr.2016.06.017] [PMID: 27349291]
[20]
Ledda C, Loreto C, Pomara C, et al. Sheep lymph-nodes as a biological indicator of environmental exposure to fluoro-edenite. Environ Res 2016; 147(147): 97-101.
[http://dx.doi.org/10.1016/j.envres.2016.01.043] [PMID: 26855127]
[21]
Rapisarda V, Rapisarda G, Vico GD, Gobbi L, Loreto C, Valentino M. Monitoring of fluoro-edenite fibre pollution through the study of sheep lymph nodes as a model of a biological indicator. Occup Environ Med 2005; 62(9): 656.
[http://dx.doi.org/10.1136/oem.2005.020727] [PMID: 16109823]
[22]
Bégin R, Rola-Pleszczynski M, Sirois P, Masse S, Nadeau D, Bureau MA. Sequential analysis of the bronchoalveolar milieu in conscious sheep. J Appl Physiol 1981; 50(3): 665-71.
[http://dx.doi.org/10.1152/jappl.1981.50.3.665] [PMID: 7251456]
[23]
May D. The anatomy of the sheep. Queensland: Queensland University Press 1970.
[24]
McConnochie K, Simonato L, Mavrides P, Christofides P, Pooley FD, Wagner JC. Mesothelioma in Cyprus: the role of tremolite. Thorax 1987; 42(5): 342-7.
[http://dx.doi.org/10.1136/thx.42.5.342] [PMID: 2821642]
[25]
Dumortier P, Rey F, Viallat JR, Broucke I, Boutin C, De Vuyst P. Chrysotile and tremolite asbestos fibres in the lungs and parietal pleura of Corsican goats. Occup Environ Med 2002; 59(9): 643-6.
[http://dx.doi.org/10.1136/oem.59.9.643] [PMID: 12205241]
[26]
Dodson RF, Williams MG Jr, Corn CJ, Brollo A, Bianchi C. Asbestos content of lung tissue, lymph nodes, and pleural plaques from former shipyard workers. Am Rev Respir Dis 1990; 142(4): 843-7.
[http://dx.doi.org/10.1164/ajrccm/142.4.843] [PMID: 2171386]
[27]
Schlesinger RB. Clearance from the respiratory tract. Fundam Appl Toxicol 1985; 5(3): 435-50.
[http://dx.doi.org/10.1016/0272-0590 (85)90091-0] [PMID: 3891478]
[28]
Tuomi T, Segerberg-Konttinen M, Tammilehto L, Tossavainen A, Vanhala E. Mineral fiber concentration in lung tissue of mesothelioma patients in Finland. Am J Ind Med 1989; 16(3): 247-54.
[http://dx.doi.org/10.1002/ajim.4700160303] [PMID: 2782313]
[29]
Biterge B, Schneider R. Histone variants: key players of chromatin. Cell Tissue Res 2014; 356(3): 457-66.
[http://dx.doi.org/10.1007/s00441-014-1862-4] [PMID: 24781148]
[30]
Choi JK, Howe LJ. Histone acetylation: truth of consequences? Biochem Cell Biol 2009; 87(1): 139-50.
[http://dx.doi.org/10.1139/O08-112] [PMID: 19234530]
[31]
Strahl BD, Allis CD. The language of covalent histone modifications. Nature 2000; 403(6765): 41-5.
[http://dx.doi.org/10.1038/47412] [PMID: 10638745]
[32]
Chadwick BP, Willard HF. Histone H2A variants and the inactive X chromosome: identification of a second macroH2A variant. Hum Mol Genet 2001; 10(10): 1101-13.
[http://dx.doi.org/10.1093/hmg/10.10.1101] [PMID: 11331621]
[33]
Barrero MJ, Sese B, Martí M, Izpisua Belmonte JC. Macro histone variants are critical for the differentiation of human pluripotent cells. J Biol Chem 2013. 31; 288(22): 16110-6.
[34]
Gaspar-Maia A, Qadeer ZA, Hasson D, et al. MacroH2A histone variants act as a barrier upon reprogramming towards pluripotency. Nat Commun 2013; 4(4): 1565.
[http://dx.doi.org/10.1038/ncomms2582] [PMID: 23463008]
[35]
Pasque V, Gillich A, Garrett N, Gurdon JB. Histone variant macroH2A confers resistance to nuclear reprogramming. EMBO J 2011; 30(12): 2373-87.
[http://dx.doi.org/10.1038/emboj.2011.144] [PMID: 21552206]
[36]
Braig M, Schmitt CA. Oncogene-induced senescence: putting the brakes on tumor development. Cancer Res 2006; 66(6): 2881-4.
[http://dx.doi.org/10.1158/0008-5472.CAN-05-4006] [PMID: 16540631]
[37]
Chen Z, Trotman LC, Shaffer D, et al. Crucial role of p53-dependent cellular senescence in suppression of Pten-deficient tumorigenesis. Nature 2005; 436(7051): 725-30.
[http://dx.doi.org/10.1038/nature03918] [PMID: 16079851]
[38]
Collado M, Serrano M. The power and the promise of oncogene-induced senescence markers. Nat Rev Cancer 2006; 6(6): 472-6.
[http://dx.doi.org/10.1038/nrc1884] [PMID: 16723993]
[39]
Michaloglou C, Vredeveld LC, Soengas MS, et al. BRAFE600-associated senescence-like cell cycle arrest of human naevi. Nature 2005; 436(7051): 720-4.
[http://dx.doi.org/10.1038/nature03890] [PMID: 16079850]
[40]
Pehrson JR, Costanzi C, Dharia C. Developmental and tissue expression patterns of histone macroH2A1 subtypes. J Cell Biochem 1997; 65(1): 107-13.
[http://dx.doi.org/10.1002/(SICI)1097-4644(199704)65:1<107:AID-JCB11>3.0.CO;2-H] [PMID: 9138085]
[41]
Serrano M, Lin AW, McCurrach ME, Beach D, Lowe SW. Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a. Cell 1997; 88(5): 593-602.
[http://dx.doi.org/10.1016/S0092-8674 (00)81902-9] [PMID: 9054499]
[42]
Leonardi R, Loreto C, Barbato E, Polimeni A, Caltabiano R, Lo Muzio L. A histochemical survey of the human temporomandibular joint disc of patients with internal derangement without reduction. J Craniofac Surg 2007; 18(6): 1429-33.
[http://dx.doi.org/10.1097/scs.0b013e31814fb72a] [PMID: 17993895]
[43]
Leonardi R, Talic NF, Loreto C. MMP-13 (collagenase 3) immunolocalisation during initial orthodontic tooth movement in rats. Acta Histochem 2007; 109(3): 215-20.
[http://dx.doi.org/10.1016/j.acthis.2007.01.002] [PMID: 17350083]
[44]
Bernstein E, Muratore-Schroeder TL, Diaz RL, et al. A phosphorylated subpopulation of the histone variant macroH2A1 is excluded from the inactive X chromosome and enriched during mitosis. Proc Natl Acad Sci USA 2008; 105(5): 1533-8.
[http://dx.doi.org/10.1073/pnas.0711632105] [PMID: 18227505]
[45]
Monteiro FL, Baptista T, Amado F, Vitorino R, Jerónimo C, Helguero LA. Expression and functionality of histone H2A variants in cancer. Oncotarget 2014; 5(11): 3428-43.
[http://dx.doi.org/10.18632/oncotarget.2007] [PMID: 25003966]
[46]
Novikov L, Park JW, Chen H, Klerman H, Jalloh AS, Gamble MJ. QKI-mediated alternative splicing of the histone variant MacroH2A1 regulates cancer cell proliferation. Mol Cell Biol 2011; 31(20): 4244-55.
[http://dx.doi.org/10.1128/MCB.05244-11] [PMID: 21844227]
[47]
Rasmussen TP, Huang T, Mastrangelo MA, Loring J, Panning B, Jaenisch R. Messenger RNAs encoding mouse histone macroH2A1 isoforms are expressed at similar levels in male and female cells and result from alternative splicing. Nucleic Acids Res 1999; 27(18): 3685-9.
[http://dx.doi.org/10.1093/nar/27.18.3685] [PMID: 10471737]
[48]
Pehrson JR, Fuji RN. Evolutionary conservation of histone macroH2A subtypes and domains. Nucleic Acids Res 1998; 26(12): 2837-42.
[http://dx.doi.org/10.1093/nar/26.12.2837] [PMID: 9611225]
[49]
Sporn JC, Kustatscher G, Hothorn T, et al. Histone macroH2A isoforms predict the risk of lung cancer recurrence. Oncogene 2009; 28(38): 3423-8.
[http://dx.doi.org/10.1038/onc.2009.26] [PMID: 19648962]
[50]
Dardenne E, Pierredon S, Driouch K, et al. Splicing switch of an epigenetic regulator by RNA helicases promotes tumor-cell invasiveness. Nat Struct Mol Biol 2012; 19(11): 1139-46.
[http://dx.doi.org/10.1038/nsmb.2390] [PMID: 23022728]
[51]
Pliatska M, Kapasa M, Kokkalis A, Polyzos A, Thanos D. The Histone Variant MacroH2A Blocks Cellular Reprogramming by Inhibiting Mesenchymal-to-Epithelial Transition. Mol Cell Biol 2018; 38(10): e00669-17.
[http://dx.doi.org/10.1128/MCB.00669-17] [PMID: 29483300]
[52]
Shu X, Pei D. The function and regulation of mesenchymal-to-epithelial transition in somatic cell reprogramming. Curr Opin Genet Dev 2014; 28(28): 32-7.
[http://dx.doi.org/10.1016/j.gde.2014.08.005] [PMID: 25173869]
[53]
Lamouille S, Xu J, Derynck R. Molecular mechanisms of epithelial-mesenchymal transition. Nat Rev Mol Cell Biol 2014; 15(3): 178-96.
[http://dx.doi.org/10.1038/nrm3758] [PMID: 24556840]
[54]
Bersaas A, Arnoldussen YJ, Sjøberg M, Haugen A, Mollerup S. Epithelial-mesenchymal transition and FOXA genes during tobacco smoke carcinogen induced transformation of human bronchial epithelial cells. Toxicol In Vitro 2016; 35(35): 55-65.
[http://dx.doi.org/10.1016/j.tiv.2016.04.012] [PMID: 27221058]
[55]
Kamp DW, Graceffa P, Pryor WA, Weitzman SA. The role of free radicals in asbestos-induced diseases. Free Radic Biol Med 1992; 12(4): 293-315.
[http://dx.doi.org/10.1016/0891-5849 (92)90117-Y] [PMID: 1577332]
[56]
Kim MC, Cui FJ, Kim Y. Hydrogen peroxide promotes epithelial to mesenchymal transition and stemness in human malignant mesothelioma cells. Asian Pac J Cancer Prev 2013; 14(6): 3625-30.
[http://dx.doi.org/10.7314/APJCP.2013.14.6.3625] [PMID: 23886156]
[57]
Rapisarda V, Loreto C, Ledda C, et al. Cytotoxicity, oxidative stress and genotoxicity induced by glass fibers on human alveolar epithelial cell line A549. Toxicol In Vitro 2015; 29(3): 551-7.
[http://dx.doi.org/10.1016/j.tiv.2014.12.010] [PMID: 25620604]
[58]
Rapisarda V, Ledda C, Ricceri V, et al. Detection of pleural plaques in workers exposed to inhalation of natural fluoro-edenite fibres. Oncol Lett 2015; 9(5): 2046-52.
[http://dx.doi.org/10.3892/ol.2015.2978] [PMID: 26137010]
[59]
Miozzi E, Rapisarda V, Marconi A, et al. Fluoro-edenite and carbon nanotubes: The health impact of ‘asbestos-like’ fibres. Exp Ther Med 2016; 11(1): 21-7. [review]
[http://dx.doi.org/10.3892/etm.2015.2894] [PMID: 26889212]
[60]
Ledda C, Pomara C, Bracci M, et al. Natural carcinogenic fiber and pleural plaques assessment in a general population: A cross-sectional study. Environ Res 2016; 150: 23-9.
[http://dx.doi.org/10.1016/j.envres.2016.05.024] [PMID: 27236568]
[61]
Rapisarda V, Ledda C, Migliore M, et al. FBLN-3 as a biomarker of pleural plaques in workers occupationally exposed to carcinogenic fibers: a pilot study. Future Oncol 2015; 11(24)(Suppl.): 35-7.
[http://dx.doi.org/10.2217/fon.15.271] [PMID: 26638921]
[62]
Ledda C, Loreto C, Matera S, et al. Early effects of fluoro-edenite: correlation between IL-18 serum levels and pleural and parenchymal abnormalities. Future Oncol 2016; 12(23s): 59-62.
[http://dx.doi.org/10.2217/fon-2016-0338] [PMID: 27669761]
[63]
Ledda C, Loreto C, Bracci M, et al. High risk of pleural plaques and parenchymal abnormalities in women living in Biancavilla (Italy). Future Oncol 2016; 12(23s): 63-5.
[http://dx.doi.org/10.2217/fon-2016-0337] [PMID: 27624550]
[64]
Rapisarda V, Salemi R, Marconi A, et al. Fluoro-edenite induces fibulin-3 overexpression in non-malignant human mesothelial cells. Oncol Lett 2016; 12(5): 3363-7.
[http://dx.doi.org/10.3892/ol.2016.5051] [PMID: 27900005]
[65]
Ledda C, Costa C, Matera S, et al. Immunomodulatory effects in workers exposed to naturally occurring asbestos fibers. Mol Med Rep 2017; 15(5): 3372-8.
[http://dx.doi.org/10.3892/mmr.2017.6384] [PMID: 28339042]
[66]
Ledda C, Caltabiano R, Loreto C, et al. Prevalence of anti-nuclear autoantibodies in subjects exposed to natural asbestiform fibers: a cross-sectional study. J Immunotoxicol 2018; 15(1): 24-8.
[http://dx.doi.org/10.1080/1547691X.2017.1415398] [PMID: 29241379]
[67]
Rapisarda V, Loreto C, Castorina S, et al. Occupational exposure to fluoro-edenite and prevalence of anti-nuclear autoantibodies. Future Oncol 2018; 14(6s): 59-62.
[http://dx.doi.org/10.2217/fon-2017-0389] [PMID: 29664352]
[68]
Ledda C, Caltabiano R, Vella F, et al. Fibulin-3 as biomarker of malignant mesothelioma. Biomarkers Med 2019; 13(10): 875-86.
[http://dx.doi.org/10.2217/bmm-2018-0285] [PMID: 31237454]
[69]
Pugnaloni A, Lucarini G, Giantomass IF, et al. In vitro study of biofunctional indicators after exposure to asbestos-like fluoro-edenite fibres. Cell Mol Biol 2007; 53(53)(Suppl.): OL965-80.
[PMID: 17695086]
[70]
Nollet F, Kools P, van Roy F. Phylogenetic analysis of the cadherin superfamily allows identification of six major subfamilies besides several solitary members. J Mol Biol 2000; 299(3): 551-72.
[http://dx.doi.org/10.1006/jmbi.2000.3777] [PMID: 10835267]
[71]
Fu Y, Lv P, Yan G, et al. MacroH2A1 associates with nuclear lamina and maintains chromatin architecture in mouse liver cells. Sci Rep 2015; 5(5): 17186.
[http://dx.doi.org/10.1038/srep17186] [PMID: 26603343]
[72]
Douet J, Corujo D, Malinverni R, et al. MacroH2A histone variants maintain nuclear organization and heterochromatin architecture. J Cell Sci 2017; 130(9): 1570-82.
[http://dx.doi.org/10.1242/jcs.199216] [PMID: 28283545]
[73]
Ledda C, Senia P, Rapisarda V. Biomarkers for early diagnosis and prognosis of malignant pleural mesothelioma: The quest goes on. Cancers (Basel) 2018; 10(6)E203
[http://dx.doi.org/10.3390/cancers10060203] [PMID: 29914087 ]

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