Login Register Cart 0
Generic placeholder image

Infectious Disorders - Drug Targets

Editor-in-Chief

ISSN (Print): 1871-5265
ISSN (Online): 2212-3989

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Evaluation of Various Diagnostic Techniques for the Diagnosis of Pulmonary and Extra Pulmonary Tuberculosis at a Tertiary Care Center in North India

Author(s): Monika Rajani* and Molay Banerjee

Volume 20, Issue 4, 2020

Page: [433 - 439] Pages: 7

DOI: 10.2174/1871526519666191011165702

Price: $65

Abstract

Introduction: Tuberculosis (TB) is a one of the main causes of mortality and morbidity worldwide. Bactec MGIT (Mycobacteria Growth Indicator Tube) system is a rapid, reliable automated system for early diagnosis of pulmonary and extra pulmonary TB in setups where purchase of expensive instruments is not possible. The present study was thus carried out to evaluate AFB microscopy, culture on Lowenstein Jensen media and micro MGIT system for early and accurate diagnosis of Tuberculosis.

Methods: A total of 280 samples were processed for direct AFB smear examination, and culture on micro MGIT and LJ media. The identification of Mycobacterium tuberculosis complex in positive cultures was done by MPT64 Ag card test (BD MGIT TBC Identification Test).

Results: Out of the processed samples, (47.1%) 132/280 were positive for Mycobacterium spp by Micro MGIT, (35%) 98/280 on LJ medium and (25.7%) 72/280 by AFB smear. A total of (48.5%) 136 samples were positive by a combination of Micro MGIT and LJ medium. Among the total positive samples (136/280), Micro MGIT was found to be positive in 97% (132/136) of samples, LJ was positive in 72% (98/136), while 52.9% (72/136) were positive by AFB smear.

Conclusion: Manual MGIT System is a simple and efficient, safe to use the diagnostic system. It does not require any expensive/special instrumentation other than the UV lamp for the detection of fluorescence. In areas with limited resources where the purchase of expensive instruments such as the MGIT 960 is out of scope, the use of manual MGIT for rapid susceptibility testing for MDR-TB could be an option. We would recommend testing MGIT 960 using first and secondline drugs to determine DST.

Keywords: Mycobacterium tuberculosis, micro MGIT, Lowenstein Jensen (LJ) media, AFB smear, MDR-TB, DST.

[1]
Tuberculosis - World Health Organization. https://www.who.int
[2]
India continues to be worst affected by tuberculosis: WHO report. https://www.livemint.com
[3]
Rao, P.; Chawla, K.; Shenoy, V.P.; Mukhopadhyay, C.; Brahmavar, V.; Kamath, A.; Mohapatra, A.K. Study of drug resistance in pulmonary tuberculosis cases in south coastal Karnataka. J. Epidemiol. Glob. Health, 2015, 5(3), 275-281.
[http://dx.doi.org/10.1016/j.jegh.2015.01.002] [PMID: 25689297]
[4]
Kwan, C.K.; Ernst, J.D. HIV and tuberculosis: a deadly human syndemic. Clin. Microbiol. Rev., 2011, 24(2), 351-376.
[http://dx.doi.org/10.1128/CMR.00042-10] [PMID: 21482729]
[5]
Muddaiah, R.K.; James, P.M.; Lingegowda, R.K. Comparative study of Smear Microscopy, Rapid Slide Culture, and Lowenstein - Jensen culture in cases of pulmonary tuberculosis in a tertiary care hospital. J. Res. Med. Sci., 2013, 18(9), 767-771.
[PMID: 24381619]
[6]
Hillemann, D.; Richter, E.; Rüsch-Gerdes, S. Use of the BACTEC Mycobacteria Growth Indicator Tube 960 automated system for recovery of Mycobacteria from 9,558 extrapulmonary specimens, including urine samples. J. Clin. Microbiol., 2006, 44(11), 4014-4017.
[http://dx.doi.org/10.1128/JCM.00829-06] [PMID: 17005737]
[7]
Mistry, Y.; Rajdev, S.; Mullan, S. Use of Cost Effective Semi-Automated (Manual/Micro) MGIT System over BACTEC 960 to Perform First Line Anti-Tuberculosis Drugs Sensitivity Testing. J. Tuberc. Res., 2016, 4, 227-234.
[http://dx.doi.org/10.4236/jtr.2016.44025]
[8]
Gholoobi, A.; Masoudi-Kazemabad, A.; Meshkat, M.; Meshkat, Z. Comparison of Culture and PCR Methods for Diagnosis of Mycobacterium tuberculosis in Different Clinical Specimens. Jundishapur J. Microbiol., 2014, 7(2) e8939
[http://dx.doi.org/10.5812/jjm.8939] [PMID: 25147673]
[9]
MGIT TM Procedure Manual - Foundation for Innovative New Diagnostics. https://www.finddx.org/wp-content/uploads/2016/02/mgit_manual_nov2006.pdf
[10]
Revisions - Becton Dickinson . https://www.bd.com/resource.?https://www.bd.com/resource.aspx? IDX=11376
[11]
Rishi, S.; Sinha, P.; Malhotra, B.; Pal, N. A comparative study for the detection of Mycobacteria by BACTEC MGIT 960, Lowenstein Jensen media and direct AFB smear examination. Indian J. Med. Microbiol., 2007, 25(4), 383-386.
[http://dx.doi.org/10.4103/0255-0857.37344] [PMID: 18087090]
[12]
Gupta, N.; Sharma, J.B.; Mittal, S.; Singh, N.; Misra, R.; Kukreja, M. Genital tuberculosis in Indian infertility patients. Int. J. Gynaecol. Obstet., 2007, 97(2), 135-138.
[http://dx.doi.org/10.1016/j.ijgo.2006.12.018] [PMID: 17362955]
[13]
Quispe, R.; Valle, G.A.; Huapaya, J.A.; Novoa, I.; Giraldo, J.; Caceres, T.; Gotuzzo, E.; Zamudio, C.; Seas, C. Manual MGIT™ system for the detection of Mycobacterium tuberculosis: insights from a high TB burden setting. Int. J. Tuberc. Lung Dis., 2016, 20(5), 605-610.
[http://dx.doi.org/10.5588/ijtld.15.0489] [PMID: 27084813]
[14]
Alcaide, F.; Benítez, M.A.; Escribà, J.M.; Martín, R. Evaluation of the BACTEC MGIT 960 and the MB/BacT systems for recovery of mycobacteria from clinical specimens and for species identification by DNA AccuProbe. J. Clin. Microbiol., 2000, 38(1), 398-401.
[PMID: 10618124]
[15]
Lu, D.; Heeren, B.; Dunne, W.M. Comparison of the automat-ed MGIT with Lowenstein Jensen media for recovery of My-cobacteria from clinical specimens. Am. J. Clin. Pathol., 2002, 118, 542-545.
[http://dx.doi.org/10.1309/65KN-2M7E-7MNN-X0TA] [PMID: 12375641]
[16]
Krüüner, A.; Yates, M.D.; Drobniewski, F.A. Evaluation of MGIT 960-based antimicrobial testing and determination of critical concentrations of first- and second-line antimicrobial drugs with drug-resistant clinical strains of Mycobacterium tuberculosis. J. Clin. Microbiol., 2006, 44(3), 811-818.
[http://dx.doi.org/10.1128/JCM.44.3.811-818.2006] [PMID: 16517859]
[17]
Palaci, M.; Ueki, S.Y.M.; Sato, D.N.; Da Silva Telles, M.A.; Curcio, M.; Silva, E.A.; Silva, M.A.E. Evaluation of mycobacteria growth indicator tube for recovery and drug susceptibility testing of Mycobacterium tuberculosis isolates from respiratory specimens. J. Clin. Microbiol., 1996, 34(3), 762-764.
[http://dx.doi.org/10.1128/JCM.34.3.762-764.1996] [PMID: 8904457]
[18]
Ardito, F.; Posteraro, B.; Sanguinetti, M.; Zanetti, S.; Fadda, G. Evaluation of BACTEC Mycobacteria Growth Indicator Tube (MGIT 960) automated system for drug susceptibility testing of Mycobacterium tuberculosis. J. Clin. Microbiol., 2001, 39(12), 4440-4444.
[http://dx.doi.org/10.1128/JCM.39.12.4440-4444.2001] [PMID: 11724858]
[19]
Rodrigues, C.; Shenai, S.; Sadani, M.; Sukhadia, N.; Jani, M.; Ajbani, K.; Sodha, A.; Mehta, A. Evaluation of the bactec MGIT 960 TB system for recovery and identification of Mycobacterium tuberculosis complex in a high through put tertiary care centre. Indian J. Med. Microbiol., 2009, 27(3), 217-221.
[http://dx.doi.org/10.4103/0255-0857.53203] [PMID: 19584501]
[20]
Stager, C.E.; Libonati, J.P.; Siddiqi, S.H.; Davis, J.R.; Hooper, N.M.; Baker, J.F.; Carter, M.E. Role of solid media when used in conjunction with the BACTEC system for mycobacterial isolation and identification. J. Clin. Microbiol., 1991, 29(1), 154-157.
[http://dx.doi.org/10.1128/JCM.29.1.154-157.1991] [PMID: 1899678]
[21]
Somoskövi, A.; Ködmön, C.; Lantos, A.; Bártfai, Z.; Tamási, L.; Füzy, J.; Magyar, P. Comparison of recoveries of mycobacterium tuberculosis using the automated BACTEC MGIT 960 system, the BACTEC 460 TB system, and Löwenstein-Jensen medium. J. Clin. Microbiol., 2000, 38(6), 2395-2397.
[http://dx.doi.org/10.1128/JCM.38.6.2395-2397.2000] [PMID: 10835013]
[22]
Huang, T.S.; Chen, C.S.; Lee, S.S.; Huang, W.K.; Liu, Y.C. Comparison of the BACTEC MGIT 960 and BACTEC 460TB systems for detection of mycobacteria in clinical specimens. Ann. Clin. Lab. Sci., 2001, 31(3), 279-283.
[PMID: 11508832]
[23]
Tortoli, E.; Cichero, P.; Piersimoni, C.; Simonetti, M.T.; Gesu, G.; Nista, D. Use of BACTEC MGIT 960 for recovery of mycobacteria from clinical specimens: multicenter study. J. Clin. Microbiol., 1999, 37(11), 3578-3582.
[http://dx.doi.org/10.1128/JCM.37.11.3578-3582.1999] [PMID: 10523555]
[24]
Chien, H.P.; Yu, M.C.; Wu, M.H.; Lin, T.P.; Luh, K.T. Comparison of the BACTEC MGIT 960 with Löwenstein-Jensen medium for recovery of mycobacteria from clinical specimens. Int. J. Tuberc. Lung Dis., 2000, 4(9), 866-870.
[PMID: 10985656]
[25]
Jarzembowski, J.A.; Young, M.B. Nontuberculous mycobacterial infections. Arch. Pathol. Lab. Med., 2008, 132(8), 1333-1341.
[PMID: 18684037]

Rights & Permissions Print Cite
Article Metrics
14
1
© 2024 Bentham Science Publishers | Privacy Policy