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当代阿耳茨海默病研究

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ISSN (Print): 1567-2050
ISSN (Online): 1875-5828

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Research Article

红细胞淀粉样蛋白β肽同工型分布在阿尔茨海默氏症和轻度认知障碍中。

卷 16, 期 11, 2019

页: [1050 - 1054] 页: 5

弟呕挨: 10.2174/1567205016666191010104355

价格: $65

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摘要

简介：我们最近发现淀粉样β（Aβ）40积聚在红细胞中，并可能导致细胞损伤，这是由假定的受损低密度（kg / L）红细胞数量增加所证明的。此外，我们提出了一种分离技术来分离和浓缩这种受损的红细胞，以进行后续分析。 目的：我们分离了高密度和低密度红细胞，并研究了阿尔茨海默病（AD），轻度认知障碍（MCI）和主观认知障碍（SCI）中Aβ肽（Aβ40，Aβ42和Aβ43）的积累模式。 方法：通过密度梯度对全血进行分级，得到两个浓缩的高浓度和假定的低密度受损红细胞分数。细胞裂解后，通过ELISA对细胞内Aβ40，Aβ42和Aβ43进行定量。 结果：在高密度和低密度红细胞中，Aβ40在MCI中的浓度最低，而在AD和SCI中则相等且较高。检测到的Aβ40含量是Aβ42的10倍，在受损的低密度红细胞中，AD和MCI中的Aβ42含量最低。 Aβ40的表达量是Aβ43的100倍，MCI受试者较轻的红细胞显示的细胞内Aβ43较SCI少。 结论：AD，MCI和SCI之间Aβ40，Aβ42和Aβ43的红细胞积累模式存在显着差异。数据必须通过更大的临床试验进行验证。然而，在红血球亚群中Aβ肽的分布具有用于诊断目的的潜力是可以成立的。

关键词: 阿尔茨海默氏症，淀粉样β40，淀粉样β42，淀粉样β43，红细胞，MCI，SCI。

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