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Current Nutrition & Food Science

Editor-in-Chief

ISSN (Print): 1573-4013
ISSN (Online): 2212-3881

Research Article

Melissopalynological, Physicochemical, Antimicrobial and Microbiological Attributes of Commercial Honeys from North West Nigeria

Author(s): Husaina M. Mazai, Sani Ibrahim, Aliyu Muhammad* and Abdulmumin Z. Abubakar

Volume 16, Issue 6, 2020

Page: [972 - 987] Pages: 16

DOI: 10.2174/1573401315666190904143858

Price: $65

Abstract

Background: Honey is used for nutritional, medicinal and industrial purposes and maintenance of the requisite quality is of great importance.

Objective: This study distinctively aimed to portray twelve randomly selected profitable honeys accessible in the North West Nigerian market with regards to floral nectar origin, physicochemical factors, antimicrobial activity and microbial safety evaluations.

Methods: Twelve (12) samples of honey sourced from diverse places in the Northwest of Nigeria were analyzed for their pollen spectra, physicochemical parameters, antimicrobial activity and microbiological safety.

Results: Dominant pollen types from the analysis were Borassus (72%), Piptadenia africana (39%), Lophira (91%) and Combretaceae (91%) for Sokoto, Jigawa, Kaduna and Katsina States honey samples, respectively. The physicochemical analysis of the samples revealed that pH was in range from 3.32 to 4.25, moisture content varied from 16.37% to 18.70%, ash content 0.23% to 0.93% and free acidity 6.83meq/Kg to 32.00meq/kg, electrical conductivity ranged from 0.13mS/cm to 1.40mS/cm, reducing sugars was 42.85% to 49.30%, sucrose content was 0.74% to 6.75%, Hydroxymethylfurfural value was 11.0mg/Kg to 80.20mg/Kg, diastase level ranged from 7.23 to 43.5 and Proline content ranged from 104.67mg/Kg to 666.67mg/Kg. The antimicrobial evaluation revealed significant zone of inhibition to E. coli, ranging from 19-38 mm at concentrations (12.5-100mg/mL) tested as compared to all other test organisms.

Conclusion: The fallouts of microbiological investigation of the samples revealed mould and bacteria, with counts less than 102CFU/g. The findings showed that the attributes of the tested honey samples maintained criteria of international standards.

Keywords: Adulteration, antimicrobial properties, honey, microbiology, physicochemical characteristics, melissopalynological.

Graphical Abstract

[1]
De Almeida-Muradian LB, Stramm KM, Horita A, Barth OM, Da Silva de Freitas A, Estevinho LM. Comparative study of the physicochemical and palynological characteristics of honey from Melipona subnitida and Apis mellifera. Int J Food Sci Technol 2013; 48(8): 1698-706.
[http://dx.doi.org/10.1111/ijfs.12140]
[2]
Gomes S, Dias LG, Moreira LL, Rodrigues P, Estevinho L. Physicochemical, microbiological and antimicrobial properties of commercial honeys from Portugal. Food Chem Toxicol 2010; 48(2): 544-8.
[http://dx.doi.org/10.1016/j.fct.2009.11.029] [PMID: 19909782]
[3]
Mandal S. DebMandal M, Pal NK, Saha K. Antibacterial activity of honey against clinical isolates of Escherichia coli, Pseudomonas aeruginosa and Salmonella enterica serovar Typhi. Asian Pac J Trop Med 2010; 3(12): 961-4.
[http://dx.doi.org/10.1016/S1995-7645(11)60009-6]
[4]
Cooper RA, Molan PC, Harding KG. The sensitivity to honey of Gram-positive Cocci of clinical significance isolated from wounds. J Appl Microbiol 2002; 93(5): 857-63.
[http://dx.doi.org/10.1046/j.1365-2672.2002.01761.x] [PMID: 12392533]
[5]
Aliyu M, Odunola OA, Farooq AD, et al. Fractionation of acacia honey affects its antioxidant potential in vitro. J Acute Dis 2012; 1(2): 115-9.
[http://dx.doi.org/10.1016/S2221-6189(13)60027-X]
[6]
Aliyu M, Odunola OA, Farooq AD, et al. Acacia Honey Modulates Cell Cycle Progression, Pro-inflammatory Cytokines and Calcium Ions Secretion in PC-3 Cell Lines. Cancer Sci Ther 2012; 4(12): 401-7.
[7]
Muhammad A, Odunola OA, Ibrahim MA, et al. Potential biological activity of acacia honey. Front Biosci Elit 2016; 1(8): 351-7
[8]
Muhammad A, Odunola OA, Gbadegesin MA, Adegoke AM, Olugbami JO, Uche NS. Modulatory role of Acacia honey from North-West Nigeria on sodium arsenite-induced clastogenicity and oxidative stress in male Wistar rats. Nat Prod Res 2015; 29(4): 321-6.
[http://dx.doi.org/10.1080/14786419.2014.940945] [PMID: 25105348]
[9]
Aliyu M, Odunola OA, Farooq AD, et al. Molecular mechanism of antiproliferation potential of Acacia honey on NCI-H460 cell line. Nutr Cancer 2013; 65(2): 296-304.
[http://dx.doi.org/10.1080/01635581.2013.756920] [PMID: 23441617]
[10]
Iglesias A, Feás X, Rodrigues S, et al. Comprehensive study of honey with protected denomination of origin and contribution to the enhancement of legal specifications. Molecules 2012; 17(7): 8561-77.
[http://dx.doi.org/10.3390/molecules17078561] [PMID: 22805507]
[11]
Elegami AA, El-Nima EI, El Tohami MS, Muddathir AK. Antimicrobial activity of some species of the family Combretaceae. Phytother Res 2002; 16(6): 555-61.
[http://dx.doi.org/10.1002/ptr.995] [PMID: 12237814]
[12]
Olaitan PB, Adeleke OE, Ola IO. Honey: a reservoir for microorganisms and an inhibitory agent for microbes. Afr Health Sci 2007; 7(3): 159-65.
[PMID: 18052870]
[13]
Louveaux JM, Vorwohl AYG. Methods of melissopalynology. Bee World 1970; 51: 125-38.
[http://dx.doi.org/10.1080/0005772X.1970.11097312]
[14]
AOAC. Association of Official Analitycal Chemists. Arlington, VA, USA: Assoc Off Anal Chem 1990; Vol. 2: pp. 1-1213.
[15]
AOAC. Official methods of analysis, association of analytical chemists. 15th ed. Washington, DC: AOAC 2000.
[16]
International Honey Commission (ICH). Harmonised Methods of the International Honey Commission 2009.
[17]
Okunlola A, Adewoyin BA, Odeku OA. Evaluation of pharmaceutical and microbial qualities of some herbal medicinal products in South Western Nigeria. Trop J Pharm Res 2007; 6(1): 661-70.
[http://dx.doi.org/10.4314/tjpr.v6i1.14644]
[18]
Basualdo C, Sgroy V, Finola MS, Marioli JM. Comparison of the antibacterial activity of honey from different provenance against bacteria usually isolated from skin wounds. Vet Microbiol 2007; 124(3-4): 375-81.
[http://dx.doi.org/10.1016/j.vetmic.2007.04.039] [PMID: 17540520]
[19]
Ghosh S, Chakraborty R, Raychaudhuri U. Determination of pHdependent antioxidant activity of palm (Borassus flabellifer) polyphenol compounds by photoluminol and DPPH methods: a comparison of redox reaction sensitivity 3 Biotech 2015; 5: 633-40
[20]
Onyenibe S, Emmanuel OB, Onyenibe S. Ameliorative effect of stem bark extract of Piptadenia africana on ethanol-induced toxicity in male Wistar rats. Der Pharm Lett 2015; 7(4): 40-6.
[21]
Ali SA, Abdulraheem OR, Sule IM, Ilyas M, Abdul K. Antimicrobial studies of aqueous extract of the leaves of Lophira Lanceolata. Res J Pharm Biol Chem Sci 2011; 2(1): 629-36.
[22]
Odeyemi AT, Adefemi SO, Adebayo AA. Antimicrobial and proximate properties of some processed honey in Ekiti 2013; ; 4: 36-43.
[23]
Omafuvbe B, Akanbi O. Microbiological and physico-chemical properties of some commercial Nigerian honey. African J Microbiol Res 2009; 3(12): 891-6.
[24]
Malika N, Mohamed F, Chakib E. Microbiological and Physico-chemical properties of Moroccan honey. Int J Agric Biol 2005; 50: 773-6.
[25]
Makhloufi C, Kerkvliet JD, D’albore GR, Choukri A, Samar R. Characterization of Algerian honeys by palynological and physico-chemical methods. Apidologie (Celle) 2010; 41: 509-21.
[http://dx.doi.org/10.1051/apido/2010002]
[26]
El Sohaimy SA, Masry SHD, Shehata MG. Physicochemical characteristics of honey from different origins. Ann Agric Sci 2015; 60(2): 279-87.
[http://dx.doi.org/10.1016/j.aoas.2015.10.015]
[27]
Miguel J, Tulipani AS, Romandini S, Bertoli E, Battino M. Contribution of honey in nutrition and human health : a review. Med J Nutrition Metab 2010; 3: 15-23.
[http://dx.doi.org/10.1007/s12349-009-0051-6]
[28]
Buba F. Physicochemical and microbiological properties of honey from North East Nigeria. Biochem Anal Biochem 2013; 2: 142.
[http://dx.doi.org/10.4172/2161-1009.1000142]
[29]
Bogdanov S, D’Arcy BR, Mossel B, Marcazzan GL. Honey quality and international regulatory standards: review by the International Honey International honey standards are specified in a European Honey. Bee World 1999; 80(2): 61-9.
[http://dx.doi.org/10.1080/0005772X.1999.11099428]
[30]
Kivrak Ş, Kivrak İ, Karababa E. Characterization of Turkish honeys regarding of physicochemical properties, and their adulteration analysis. Food Sci Technol 2016; 37(1): 1-10.
[http://dx.doi.org/10.1590/1678-457x.07916]
[31]
Adenekan MO, Amusa NA, Okpeze VE, Owosibo AO. Nutritional and microbiological components of honey samples obtained from Ogun State, Southwestern Nigeria. Eur J Sustain Dev 2012; 1(2): 271-86.
[http://dx.doi.org/10.14207/ejsd.2012.v1n2p271]
[32]
Basson NJ, Grobler SR. Antimicrobial activity of two South African honeys produced from indigenous Leucospermum cordifolium and Erica species on selected micro-organisms. BMC Complement Altern Med 2008; 8: 41.
[http://dx.doi.org/10.1186/1472-6882-8-41] [PMID: 18627601]
[33]
Adeonipekun PA, Adeniyi TA, Eden D. Antimicrobial properties and melissopalynology, proximate and elemental analyses of honey samples from three different ecozones in Nigeria. Not Sci Biol 2016; 8(3): 326-33.
[http://dx.doi.org/10.15835/nsb839844]

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