Login Register Cart 0
Generic placeholder image

Current Radiopharmaceuticals

Editor-in-Chief

ISSN (Print): 1874-4710
ISSN (Online): 1874-4729

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Preparation and Biological Evaluation of 67Gallium- Labeled Iranian Hemiscorpius Lepturus Scorpion Venom

Author(s): Amir Jalali*, Mona Haddad Zahmatkesh, Amir Reza Jalilian, Amir Taheri Borujeni and Behrouz Alirezapour

Volume 13, Issue 2, 2020

Page: [99 - 106] Pages: 8

DOI: 10.2174/1874471012666190828155227

Abstract

Background: The Hemiscorpius lepturus (H. lepturus) is a deadly scorpion species living in the southern Iran.

Objective: H. lepturus induces delayed toxicity symptoms and understanding the long term biodistribution/ biokinetic of the venom is of great interest in toxicology.

Methods: A Ga-67 labeled venom was prepared using a DOTA -conjugated venom followed by radiolabeling using 67GaCl3 at 40°C for 90 min. The purification of the radiolabeled venom was performed using size exclusion-chromatography (radiochemical purity 71%). The radiolabeled venom was stable in the final solution in the presence of human serum at 37°C for 72 hours. The tissue distribution was studied in blood, heart, liver, spleen, muscle, brain, kidney, intestine and skin tissues at the intervals of 1, 4, 24, 48 and 72 hours using tissue counting and SPECT imaging.

Results: The radiolabeled venom mixture obtained with an estimated molar activity of 0.52 MBq/μg. The main accumulation tissues during the first 72 hours were kidneys, blood, liver, intestines, stomach and skin, respectively. Therefore, it is likely that H. lepturus’ clinical effects and renal toxicity are primary and caused by direct effects of the H. lepturus venom.

Conclusion: The results have largely shown the direct clinical effects on the studied tissues during the 72-hour period and antivenom administration can strongly alleviate the toxicity effects as early as 72 hours in the management of the patients.

Keywords: Hemiscorpius lepturus, scorpion venom, gallium-67, radiolabeling, SPECT, 67Ga-DOTA-HLV.

« Previous Next »
Graphical Abstract

[1]
Jalali, A.; Rahim, F. Epidemiological review of scorpion envenomation in iran. Iran. J. Pharm. Res., 2014, 13(3), 743-756.
[PMID: 25276176]
[2]
Jalali, A.; Pipelzadeh, M.H.; Taraz, M.; Khodadadi, A.; Makvandi, M.; Rowan, E.G. Serum TNF-α levels reflect the clinical severity of envenomation following a Hemiscorpius lepturus sting. Eur. Cytokine Netw., 2011, 22(1), 5-10.
[PMID: 21411417]
[3]
Alirezapoura, B.; Jalilian, A.R.; Bolourinovin, F.; Moradkhani, S. Production and quality control of [67Ga]-DOTA-trastuzumab for radioimmunoscintigraphy. Iranian J. Pharmceutical Res., 2013, 12(2), 355-366.
[4]
Jalilian, A.R.; Mirsadeghi, L.; Haji-Hosseini, R. Preparation and biodistribution of [67Ga]-DTPA-rituximab in normal rats. J. Radioanal. Nucl. Chem., 2007, 274, 175.
[http://dx.doi.org/10.1007/s10967-006-6906-0]
[5]
Tornesello, A.L.; Buonaguro, L.; Tornesello, M.L.; Buonaguro, F.M. New insights in the design of bioactive peptides and chelating agents for imaging and therapy in oncology. Molecules, 2017, 22(8) E1282
[http://dx.doi.org/10.3390/molecules22081282] [PMID: 28767081]
[6]
Jalilian, A.R.; Shanehsazzadeh, S.; Akhlaghi, M.; Garoosi, J.; Rajabifar, S.; Tavakoli, M.B. Preparation and evaluation of [67Ga]-DTPA-gonadorelin in normal rats. J. Radioanal. Nucl. Chem., 2008, 278(1), 123-129.
[http://dx.doi.org/10.1007/s10967-007-7241-9]
[7]
Radmanesh, M. Cutaneous manifestations of the Hemiscorpius lepturus sting: a clinical study. Int. J. Dermatol., 1998, 37(7), 500-507.
[http://dx.doi.org/10.1046/j.1365-4362.1998.00386.x] [PMID: 9679690]
[8]
Radmanesh, M. Clinical study of Hemiscorpius lepturus sting in Iran: a clinical study. Int. J. Trop. Med. Hyg., 1990, 93, 500-507.
[9]
Pipelzadeh, M.H.; Jalali, A.; Taraz, M.; Pourabbas, R.; Zaremirakabadi, A. An epidemiological and a clinical study on scorpionism by the Iranian scorpion Hemiscorpius lepturus. Toxicon, 2007, 50(7), 984-992.
[http://dx.doi.org/10.1016/j.toxicon.2007.07.018] [PMID: 17854855]
[10]
Seyedian, R.; Jalali, A.; Babaee, M.H.; Pipelzadeh, M.H.; Rezaee, S. A biodistribution study of hemiscorpius lepturus scorpion venom and available polyclonal antivenom in rats J Venom. Anim. Toxins incl. Trop, 18(4)2012, 18(4), 375-383
[11]
El Hafny, B.; Chgoury, F. Adil, N.; Cohen, N.; Hassar, M. Intraspecific variability and pharmacoki-netic characteristics of Androctonus mauretanicus mauretanicus scorpion venom. Toxicon, 2002, 40(11), 1609-1616.
[http://dx.doi.org/10.1016/S0041-0101(02)00178-2] [PMID: 12419512]
[12]
Nunan, E.A.; Arya, V.; Hochhaus, G.; Cardoso, V.N.; Moraes-Santos, T. Age effects on the pharma-cokinetics of tityustoxin from Tityus serrulatus scorpion venom in rats. Braz. J. Med. Biol. Res., 2004, 37(3), 385-390.
[http://dx.doi.org/10.1590/S0100-879X2004000300016] [PMID: 15060708]
[13]
Hammoudi, D.; Lefort, G.; Rougeot, C.; Bon, C.; Laraba, F.; Choumet, V. Toxicokinetic and toxico-dynamic analyse of Androctonus austrslis hector venom in rats. Toxicol. Appl. Pharmacol., 2007, 218, 205-214.
[http://dx.doi.org/10.1016/j.taap.2006.11.003] [PMID: 17198719]
[14]
Revelo, M.P.; Bambirra, E.A.; Ferreira, A.P.; Diniz, C.R.; Chávez-Olórtegui, C. Body distribution of Tityus serrulatus scorpion venom in mice and effects of scorpion antivenom. Toxicon, 1996, 34(10), 1119-1125.
[http://dx.doi.org/10.1016/0041-0101(96)00074-8] [PMID: 8931252]
[15]
Jalali, A.; Seyedain, R.; Babaee, M.H.; Pipelzadeh, M.H.; Rezaee, S. A Biodistribution study of Hemiscorpius Lepturus Scorprion Venom and available Polyconal antivenom in rats. J. Venom. Anim. Toxins Incl. Trop. Dis., 2012, 18, 375-383.
[http://dx.doi.org/10.1590/S1678-91992012000400005]
[16]
Jalali, A. Bavarsad –Omidian, N.; Babaei, M.; Najafzadeh, H.; Rezaie, S. The pharmacokinetics of Hemiscorpius lepturus scorpion venom and Rezi antivenom following intrumuscular administration in rat. J. Venom Res., 2012, 3, 1-6.
[PMID: 22826800]
[17]
Jalali, A.; Moazen, S.; Babaee, M.; Dadashzade, S.; Droudi, A. The pharmacokinetics of Iranian scorpion Odonthubuthus doriae venom and the available antivenom. J. Venom Res., 2010, 1, 48-53.
[PMID: 21544182]
[18]
Al-Asmari, A.K.; Al-Saif, A.A. Scorpion sting syndrome in a general hospital in Saudi Arabia. Saudi Med. J., 2004, 25(1), 64-70.
[PMID: 14758383]
[19]
Borchani, L.; Sassi, A.; Shahbazzadeh, D.; Strub, J.M.; Tounsi-Guetteti, H.; Boubaker, M.S.; Akbari, A.; Van Dorsselaer, A.; El Ayeb, M. Heminecrolysin, the first hemolytic dermonecrotic toxin purified from scorpion venom. Toxicon, 2011, 58(1), 130-139.
[http://dx.doi.org/10.1016/j.toxicon.2011.05.016] [PMID: 21658401]

Rights & Permissions Print Cite
Article Metrics
19
3
© 2024 Bentham Science Publishers | Privacy Policy