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Current Bioactive Compounds

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ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

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Research Article

Rapid Characterization of Quorum Sensing Inhibitory Molecules from Garcinia indica Choisy Seed Methanol Extract by GC-MS Analysis

Author(s): Rajesh P. Shastry* and Mohammed Aman

Volume 16, Issue 6, 2020

Page: [887 - 891] Pages: 5

DOI: 10.2174/1573407215666190408120140

Price: $65

Abstract

Background: Bacteria communicate by producing small signaling molecules to maintain threshold cell density. Quorum sensing inhibition is an effective and most potent approach for the prevention of cell to cell communication.

Methods: In the present study, the methanol extract of Garcinia indica was tested for anti-quorum sensing activity using Chromobacterium violaceum as a biosensor. The extract inhibited violacein production in biosensor strain without affecting the growth of the bacteria and this was corroborated by bioautography assay.

Results: The bioactive fraction was analysed by the aid of GC-MS revealed that, the presence of coumarin (7-hydroxy-3-(1,1-dimethylprop-2-enyl) coumarin) and eugenol (1,2-dimethoxy-4-(1-methoxy-1- propenyl) benzene) derivatives as anti-quorum sensing molecules.

Conclusion: A detailed investigation is required to study the mechanism of action involved in control of quorum sensing signals and possible applications.

Keywords: Anti-quorum sensing, coumarin, eugenol, G. indica, plant seed extract, biosensor.

Graphical Abstract

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