Antimicrobial Activity of Different Antimicrobial Peptides (AMPs) Against Clinical Methicillin-resistant Staphylococcus aureus (MRSA)

Title:Antimicrobial Activity of Different Antimicrobial Peptides (AMPs) Against Clinical Methicillin-resistant Staphylococcus aureus (MRSA)

Volume: 18 Issue: 24

Author(s): Eleonora Ciandrini, Gianluca Morroni, Daniela Arzeni, Wojciech Kamysz, Damian Neubauer, Elzbieta Kamysz, Oscar Cirioni, Lucia Brescini, Wally Baffone and Raffaella Campana*

Affiliation:

• Department of Biomolecular Sciences, Division of Toxicological, Hygiene and Environmental Sciences, University of Urbino Carlo Bo, Urbino,Italy

Keywords: AMPs, Methicillin-resistant, Staphylococcus aureus, Clinical isolates, Antimicrobial activity, Time-kill.

Abstract: Background: Antimicrobial research is being focused to look for more effective therapeutics against antibiotic-resistant infections caused by methicillin-resistant Staphylococcus aureus (MRSA). In this direction, antimicrobial peptides (AMP) appear as promising tool.

Objectives: This study evaluated the antimicrobial activity of different AMPs (Citropin 1.1, Temporin A, Pexiganan, CA(1–7)M(2–9)NH2, Pal-KGK-NH2, Pal-KKKK-NH2, LL-37) against human MRSA clinical isolates.

Methods: The Minimum Inhibitory Concentration (MIC) was assessed for each AMP; then, the most active ones (Citropin 1.1, Temporin A, CA(1–7)M(2–9)NH2 and Pal-KGK-NH2) were tested against selected MRSA strains by time-kill studies.

Results: The lowest MIC value was observed for Pal-KGK-NH2 (1 µg/ml), followed by Temporin A (4- 16 µg/ml), CA(1–7)M(2–9)NH2 (8-16 µg/ml) and Citropin 1.1 (16-64 µg/ml), while higher MICs were evidenced for LL-37, Pexiganan and Pal-KKKK-NH2 (> 128 µg/ml). In time-kill experiments, Citropin 1.1 and CA(1-7)M(2-9)NH2 showed a relatively high percentage of growth inhibition (>30 %) for all the tested MRSA clinical isolates, with a dose-dependent activity resulting in the highest percentage of bacterial growth inhibition (89.39%) at 2MIC concentration.

Conclusion: Overall, our data demonstrated the potential of some AMPs against MRSA isolates, such as Citropin 1.1 and CA(1-7)M(2-9)NH2, that represents a promising area of development for different clinical applications.

Cite this article as:

Ciandrini Eleonora , Morroni Gianluca , Arzeni Daniela , Kamysz Wojciech , Neubauer Damian , Kamysz Elzbieta , Cirioni Oscar , Brescini Lucia , Baffone Wally and Campana Raffaella *, Antimicrobial Activity of Different Antimicrobial Peptides (AMPs) Against Clinical Methicillin-resistant Staphylococcus aureus (MRSA), Current Topics in Medicinal Chemistry 2018; 18 (24) . https://dx.doi.org/10.2174/1568026618666181022140348