Login Register Cart 0
Generic placeholder image

Current Pharmaceutical Analysis

Editor-in-Chief

ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

Back
Research Article

Pharmacokinetic Study of Main Active Components of Rumex nepalensis Spreng Extract in Rats Plasma by UPLC-MS/MS

Author(s): Jin Wang, Yang Chu*, Xiao Li, Navaneethakrishnan Polachi, Xue-ying Yan*, Wei Li and Shui-ping Zhou

Volume 15, Issue 4, 2019

Page: [371 - 378] Pages: 8

DOI: 10.2174/1573412914666180214130457

Price: $65

Abstract

Background: The Rumex nepalensis Spreng (RNS) is a traditional Chinese medicine containing rich anthraquinones. However, through proper investigation we have found that there were no reports on the pharmacokinetics of RNS extract in rats.

Objective: We study on the pharmacokinetic behaviors of emodin, chrysophanol and physcion after oral administration of RNS extract in rat to achieve a better understanding of further clinical application and conduct the preparation development of the herb.

Methods: In the present study, a sensitive and rapid ultra-fast liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed and validated to determine the three anthraquinones such as chrysophanol, emodin and physcion in rat plasma along with danthron as the internal standard (IS). The analytes and IS were separated on an Acquity UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 µm) by using the mobile phase of water with 3 mM ammonium acetate and acetonitrile as gradient elution at a flow rate of 0.4 mL min -1. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization (ESI) by multiple reactions monitoring (MRM) of the transitions at m/z 253.1 → 225.0 for chrysophanol, 269.0 → 224.9 for emodin, 282.7→ 240.0 for physcion and m/z 239.0 → 211.0 for IS. The limit of detection and lower limit of quantification were both 2 ng mL -1 in rat plasma.

Results: Good linearity of this method was obtained in the range of 2-1000 ng mL -1 , and the correlation coefficient was greater than 0.990. According to regulatory guidelines, the established method was fully validated, and the results were within acceptable limits.

Conclusion: The validated method was successfully applied into a pharmacokinetic study of orally administered RNS extract in rats.

Keywords: Rumex nepalensis Spreng, UPLC-MS/MS, pharmacokinetics, anthraquinones, rat, plasma.

« Previous Next »
Graphical Abstract

[1]
Vasas, A.; Orbángyapai, O.; Hohmann, J. The Genus Rumex: Review of traditional uses, phytochemistry and pharmacology. J. Ethnopharmacol., 2015, 175, 198-228.
[2]
Editorial Committee of Flora of China, Chinese Academy of Sciences. Flora of China; Science Press: Beijing, China, 1997, Volume. 44, pp. 181-121.
[3]
Ghosh, L.; Gayen, J.R.; Sinha, S.; Pal, S.; Pal, M.; Saha, B.P. Antibacterial efficacy of Rumex nepalensis spreng roots. Phytother. Res., 2003, 17(5), 558-559.
[4]
Fairbairn, J.W.; El-muhtadi, F.J. Chemotaxonany athraquinones in Rumex. Phytochemistry, 1972, 11, 263-268.
[5]
Gautam, R.; Karkhile, K.V.; Bhutani, K.K.; Jachak, S.M. Anti-inflammatory, cyclooxygenase (COX)-2, COX-1 inhibitory, and free radical scavenging effects of Rumex nepalensis. Planta Med., 2010, 76(14), 1564-1569.
[6]
Shia, C.S.; Juang, S.H.; Tsai, S.Y.; Chang, P.H.; Kuo, S.C.; Hou, Y.C.; Chao, P.D. Metabolism and pharmacokinetics of anthraquinones in Rheum palmatum in rats and ex vivo antioxidant activity. Planta Med., 2009, 75(13), 1386-1392.
[7]
Zhang, K.; Liu, J.; You, X.; Kong, P.; Song, Y.; Cao, L.; Yang, S.; Wang, W.; Fu, Q.; Ma, Z. P2×7as a new target for chrysophanol to treat lipopolysaccharide-induced depression in mice. Neurosci. Lett., 2015, 613, 60-65.
[8]
Wijesekara, I.; Zhang, C.; Van, T.Q.; Vo, T.S.; Li, Y.X.; Kim, S.K. Physcion from marine-derived fungus Microsporum sp. induces apoptosis in human cervical carcinoma HeLa cells. Microbiol. Res., 2014, 169(4), 255-261.
[9]
Yang, C.; Wang, S.; Guo, X.; Sun, J.; Liu, L.; Wu, L. Simultaneous determination of seven anthraquinones in rat plasma by Ultra High Performance Liquid Chromatography-tandem Mass Spectrometry and pharmacokinetic study after oral administration of Semen Cassiae extract. J. Ethnopharmacol., 2015, 169, 305-313.
[10]
Wu, W.; Yan, R.; Yao, M.; Zhan, Y.; Wang, Y. Pharmacokinetics of anthraquinones in rat plasma after oral administration of a rhubarb extract. Biomed. Chromatogr., 2014, 28(4), 564.
[11]
Gautam, R.; Srivastava, A.; Jachak, S.M. Simultaneous determination of naphthalene and anthraquinone derivatives in Rumex nepalensis Spreng. Roots by HPLC: comparison of different extraction methods and validation. Phytochem. Anal., 2011, 22(2), 153.
[12]
Wang, Y.; Xu, C.; Wang, P.; Lin, X.; Yang, Y.; Li, D.; Li, H.; Wu, X.; Liu, H. Pharmacokinetic comparisons of different combinations of Shaoyao-Gancao-Decoction in rats: simultaneous determination of ten active constituents by HPLC-MS/MS. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2013, 932(1), 76.
[13]
Wu, Y.P.; Meng, X.S.; Bao, Y.R.; Wang, S. Pharmacokinetic study of four flavones of Glycyrrhiza in rat plasma using HPLC-MS. J. Ethnopharmacol., 2013, 148(1), 266-270.
[14]
Shi, J.; Zheng, L.; Lin, Z.; Hou, C.; Liu, W.; Yan, T.; Zhu, L.; Wang, Y.; Lu, L.; Liu, Z. Study of pharmacokinetic profiles and characteristics of active components and their metabolites in rat plasma following oral administration of the water extract of Astragali radix using UPLC-MS/MS. J. Ethnopharmacol., 2015, 169, 183-194.
[15]
Jiao, Y.; Zuo, Y. Ultrasonic extraction and HPLC determination of anthraquinones, aloe-emodine, emodine, rheine, chrysophanol and physcione, in roots of Polygoni multiflori. Phytochem. Anal., 2009, 20(4), 272-278.
[16]
US Food and Drug Administration. (2001). Guidance for industry bioanalytical method evaluation. Rockville(MD): US Department of Health and Human Services, CDER and CVM..
[17]
Shia, C.S.; Tsai, S.Y.; Lin, J.C.; Li, M.L.; Ko, M.H.; Chao, P.D.; Huang, Y.C.; Hou, Y.C. Steady-state pharmacokinetics and tissue distribution of anthraquinones of Rhei rhizoma in rats. J. Ethnopharmacol., 2011, 137(3), 1388-1394.
[18]
Zhang, L.; Chang, J.H.; Zhang, B.Q.; Liu, X.G.; Liu, P.; Xue, H.F.; Liu, L.Y.; Fu, Q.; Zhu, M.; Liu, C.Z. The pharmacokinetic study on the mechanism of toxicity attenuation of rhubarb total free anthraquinone oral colon-specific drug delivery system. Fitoterapia, 2015, 104(15), 86.
[19]
Zhu, Z.; Zhao, L.; Liu, X.; Chen, J.; Zhang, H.; Zhang, G.; Chai, Y. Comparative pharmacokinetics of baicalin and wogonoside by liquid chromatography-mass spectrometry after oral administration of Xiaochaihu tang and Radix scutellariae extract to rats. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2010, 878(24), 2184-2190.
[20]
Li, P.; Lu, Q.; Jiang, W.; Pei, X.; Sun, Y.; Hao, H.; Hao, K. Pharmacokinetics and pharmacodynamics of Rhubarb anthraquinones extract in normal and disease rats. Biomed. Pharmacother., 2017, 91, 425.
[21]
Guan, X.; Wang, X.; Yan, K.; Chu, Y.; Li, S.; Li, W.; Yan, X.; Ma, X.; Zhou, S.; Sun, H.; Liu, C. UFLC-MS/MS determination and pharmacokinetic studies of six Saikosaponins in rat plasma after oral administration of Bupleurum Dropping Pills. J. Pharm. Biomed. Anal., 2016, 124, 288-293.

Rights & Permissions Print Cite
Article Metrics
33
3
© 2024 Bentham Science Publishers | Privacy Policy