The Use of Fluorescence Polarization Assays for the Detection of Infectious Diseases

Title: The Use of Fluorescence Polarization Assays for the Detection of Infectious Diseases

Volume: 6 Issue: 3

Author(s): Michael E. Jolley and Mohammad S. Nasir

Affiliation:

Keywords: Fluorescence Polarization, polysaccharides, enterohemorrhagic, Salmonella spp, Brucella spp

Abstract: Fluorescence Polarization Assays (FPAs) have been shown to have great utility in the detection of infectious diseases. Examples are presented of the use of O-polysaccharides (OPSs) for the detection of antibodies in serum, whole milk and whole blood to gram negative organisms (Brucella spp., Salmonella spp.). The use of proteins and peptides are also described for the detection of Mycobacterium bovis and Equine Infectious Anemia Virus. Fluorescence Polarization Inhibition Assays (FPIAs) are discussed for the specific and sensitive detection and quantitation of Salmonella spp. cells from culture. An example of the detection of enterohemorrhagic E. coli (EHECS) by Strand Displacement Amplification (SDA), coupled with FP, down to the single cell level, within thirty minutes, is described.

Cite this article as:

Jolley E. Michael and Nasir S. Mohammad, The Use of Fluorescence Polarization Assays for the Detection of Infectious Diseases, Combinatorial Chemistry & High Throughput Screening 2003; 6 (3) . https://dx.doi.org/10.2174/138620703106298419