DNA损伤复合物：利用高通量测序阐明其多效性

Title:DNA Damage-inducing Compounds: Unraveling their Pleiotropic Effects Using High Throughput Sequencing

Volume: 24 Issue: 15

关键词: DNA损伤，化学生物学，测序，下一代测序，小分子，药物。

摘要: Compounds causing DNA damage have been used widely in molecular biology and some are used as therapeutic agents in cancer therapy. In most cases, their cellular response is pleiotropic, making it challenging to develop these agents efficiently for potential therapeutic use. Furthermore, this means that such compounds can also affect healthy tissues, which is a major drawback for the use in therapy. Thus, dissecting and understanding not only their molecular mode of action, but also identifying all their cellular targets is critical. With the advent of high throughput DNA sequencing technologies our understanding of the genomic targets of such compounds has increased significantly over recent years. This review gives an overview of some well-studied DNA-damaging agents and dissects what is known about their molecular mode of action, their cellular response and use in clinical settings. It then describes how high throughput-sequencing approaches can be used (a) to study DNAdamaging compounds and (b) to gain insight into their biological activity in vivo.

Cite this article as:

DNA Damage-inducing Compounds: Unraveling their Pleiotropic Effects Using High Throughput Sequencing, Current Medicinal Chemistry 2017; 24 (15) . https://dx.doi.org/10.2174/0929867324666170124143710