Determination of Cd2+, Cr3+, Cu2+ and Zn2+ in Traditional Chinese Medicine Shuanghuanglian Oral Liquid by High Performance Capillary Electrophoresis

Title:Determination of Cd2+, Cr3+, Cu2+ and Zn2+ in Traditional Chinese Medicine Shuanghuanglian Oral Liquid by High Performance Capillary Electrophoresis

Volume: 13 Issue: 3

Author(s): Feng Luan*, Yuxi Lu, Lili Tang and Huitao Liu

Affiliation:

• College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005,China

Keywords: High perfomance capillary electrophoresis, heavy metal ions, indirect UV method, shuanghuanglian oral liquid, ditional chinese medicine, calibration curves.

Abstract: Background: The safety and level of heavy metal ions in the traditional Chinese medicine has become an important topic. Most of the metal ions are carcinogens and lead to serious health concerns by producing free radicals. It is of great significance to develop simple and effective methods to determine heavy metal ions. The aim of this paper is to develop a CE-indirect UV method for the analysis of the four heavy metal ions for Chinese medicine Shuanghuanglian oral liquid.

Methods: The alternative CE method for separation and test of metal ions was developed in this paper, which can help control the safety and level of heavy metal ions in traditional Chinese medicine Shuanghuanglian oral liquid. And the developed HPCE method showed superior analytical performance, including high sensitivity and good selectivity.

Results: The experimental conditions of the CE separation method were: 5s hydrodynamic injection at 0.5 psi, 20 kV applied voltage, and UV detection at 218 nm. The results show that the migration time of all the four heavy metal ions was less than 6 min. The calibration curves for four heavy metal ions were linear in the range of 0.5-35 μg mL-1 with linear correlation coefficients of 0.9984-0.9999. The limits of detection were in the range of 0.08-0.61 μg mL-1 along with the recoveries of 93.0% to 109.5%.

Conclusion: A simple, rapid and sensitive HPCE method has been developed to separate four heavy metal ions including Cd2+, Cr3+, Cu2+ and Zn2+ in traditional Chinese medicine Shuanghuanglian oral liquid. The comprehensive analysis performance is satisfactory. The proposed HPCE method provides shorter analysis time and lower detection limit. The results demonstrated that the developed CE method can be potentially applied as an good alternative for quality control of TCM.

Cite this article as:

Luan Feng*, Lu Yuxi, Tang Lili and Liu Huitao, Determination of Cd2+, Cr3+, Cu2+ and Zn2+ in Traditional Chinese Medicine Shuanghuanglian Oral Liquid by High Performance Capillary Electrophoresis, Current Analytical Chemistry 2017; 13 (3) . https://dx.doi.org/10.2174/1573412912666161013144007

DOI https://dx.doi.org/10.2174/1573412912666161013144007	Print ISSN 1573-4110
Publisher Name Bentham Science Publisher	Online ISSN 1875-6727