Optimal Enzymes for Single-Molecule Sequencing

Title: Optimal Enzymes for Single-Molecule Sequencing

Volume: 5 Issue: 1

Author(s): Susanne Brakmann

Affiliation:

Keywords: dna sequencing, single-molecule detection, dna polymerase, exonuclease, fluorescence

Abstract: Much research effort has been made to realize a single-molecule sequencing. Central to this project are two enzymatic tasks that challenged several biochemists during the last years. They searched for polymerases for copying a DNA target into completely fluorophore-labeled DNA as well as for handling this new DNA derivative. Furthermore, they studied exonucleases for the sequential hydrolysis of completely dye-labeled DNA and explored optimal conditions for future application on the single-molecule level. In this article, the recent advances will be summarized from the biochemists point of view.

Cite this article as:

Brakmann Susanne, Optimal Enzymes for Single-Molecule Sequencing, Current Pharmaceutical Biotechnology 2004; 5 (1) . https://dx.doi.org/10.2174/1389201043489611