Loteprednol Etabonate Nanoparticles: Optimization via Box-Behnken Design Response Surface Methodology and Physicochemical Characterization

Title:Loteprednol Etabonate Nanoparticles: Optimization via Box-Behnken Design Response Surface Methodology and Physicochemical Characterization

Volume: 14 Issue: 5

Author(s): Abhishek K. Sah and Preeti K. Suresh*

Affiliation:

• University Institute of Pharmacy, Faculty of Technology, Pt. Ravishankar Shukla University, Raipur-492010, Chhattisgarh,India

Keywords: Box-behnken design, corticosteroid, loteprednol etabonate, nanoparticle, optimization, ocular delivery, PLGA, surface response methodology.

Abstract: Background: Abstract: The objective of the present work was to prepare and optimize the loteprednoletabonate (LE) loaded poly (D,L-lactide co-glycolide) (PLGA) polymer based nanoparticle carrier. The review on recent patents (US9006241, US20130224302A1, US2012/0028947A1) assisted in the selection of drug and polymer for designing nanoparticles for ocular delivery applications.

Methods: The nanoparticles were prepared by solvent evaporation followed by high speed homogenization. Biodegradable polymer PLGA (50:50) grade was utilized to develop various formulations with different drug:polymer ratio. A Box-Behnken design with 33 factorial design was selected for the present study and 17 runs were carried out in totality. The influence of various process variables (viz., polymer concentration, homogenization speed and sonication time) on the characteristics of nanoparticles including the in vitro drug release profile were studied.

Results: The nanoparticulate formulations were evaluated for mean spherical diameter, polydispersity index (PDI), zeta potential, surface morphology, drug entrapment and in-vitro drug release profile. The entrapment efficiency, drug loading and mean particle size were found to be 96.31±1.68 %, 35.46±0.35 % and 167.6±2.1 nm respectively.

Conclusion: The investigated process and formulation variables were found to have significant effect on the particle size, drug loading (DL), entrapment efficiency (EE), and in vitro drug release profile. A biphasic in vitro drug release profile was apparent from the optimized nanoparticles (NPs) for 24 hours.

Cite this article as:

Sah K. Abhishek and Suresh K. Preeti*, Loteprednol Etabonate Nanoparticles: Optimization via Box-Behnken Design Response Surface Methodology and Physicochemical Characterization, Current Drug Delivery 2017; 14 (5) . https://dx.doi.org/10.2174/1567201813666160801125235

DOI https://dx.doi.org/10.2174/1567201813666160801125235	Print ISSN 1567-2018
Publisher Name Bentham Science Publisher	Online ISSN 1875-5704