Killing the Messenger: Antisense DNA and siRNA

Title: Killing the Messenger: Antisense DNA and siRNA

Volume: 5 Issue: 8

Author(s): M. Nesterova and Y. S. Cho-Chung

Affiliation:

Keywords: gene therapy, gene silencing, oligonucleotides, antisense, rna interference

Abstract: Among the technologies available for gene knockdown RNase H-dependent antisense oligonucleotides and RNAi are very popular. Both offer specificity and efficient knockdown of the genes; both are useful tools to study gene functions. Antisense and RNAi methods share many practical problems such as site selection, toxicity at high concentration, and the difficulty of transfection in certain cell types. We will focus in this review on the most important issues in the development of both methods and their possible use in gene-silencing therapy.

Cite this article as:

Nesterova M. and Cho-Chung S. Y., Killing the Messenger: Antisense DNA and siRNA, Current Drug Targets 2004; 5 (8) . https://dx.doi.org/10.2174/1389450043345137