Compared Chemical Activity and Effects on Cell Viability of Various Antioxidant Compounds

Title:Compared Chemical Activity and Effects on Cell Viability of Various Antioxidant Compounds

Volume: 13 Issue: 2

Author(s): Céline Clayeux, Eric Marchioni, Remmelt Van der Werf, Stéphanie Dal, Séverine Sigrist, Damien Steyer and Saïd Ennahar*

Affiliation:

• Equipe de Chimie Analytique des Molécules BioActives, UMR 7178 – IPHC, Université de Strasbourg - Faculté de Pharmacie 74, route du Rhin, 67400 Illkirch,France

Keywords: Oxidative stress, bioactive compounds, antioxidant, pro-oxidant, β pancreatic cells, ABTS.

Abstract: Background: Oxidative stress plays a major role in the development of several diseases. A healthy food diet, supplying the organism with antioxidants, is generally believed to help prevent this stress.

Methods: With the aim of screening several antioxidants commonly found in food, a method using β pancreatic cells and based on the reduction of a tetrazolium sodium salt into colored formazan in the presence of antioxidant compounds was used. In parallel, the same compounds were investigated using a chromatographic online antioxidant detection system (Coads) based on the detection of the reduced form of the radical cation 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS^•+) which is first allowed to react online with the eluting compounds.

Results: The obtained data suggested that most phenolic compounds possessed both antioxidant and prooxidant properties. Gallic acid, in particular, showed among the highest antioxidant activities with the Coads method (322±46 μM Trolox equivalent), but caused a decrease in cell viability (-25%).

Conclusion: Moreover, when screening natural compounds for their antioxidant activity, combining both chemical and biological methods is a more targeted approach compared with the single use of either method.

Cite this article as:

Clayeux Céline, Marchioni Eric, Werf Van der Remmelt, Dal Stéphanie, Sigrist Séverine, Steyer Damien and Ennahar Saïd*, Compared Chemical Activity and Effects on Cell Viability of Various Antioxidant Compounds, Current Analytical Chemistry 2017; 13 (2) . https://dx.doi.org/10.2174/1573411012666160213002321

DOI https://dx.doi.org/10.2174/1573411012666160213002321	Print ISSN 1573-4110
Publisher Name Bentham Science Publisher	Online ISSN 1875-6727