Abstract
Neem (Azadirachta indica) leaves have been widely proposed as a traditional medicine and many bioactive compounds have been isolated and characterized. Taking into account the high variability of the constituents content, which is influenced by many factors such as the environment and the crop production technology, a proper and fast analytical method is important for monitoring the chemical composition of the product. Here, we report, for the first time, the NMR-based metabolic fingerprinting of Neem leaves extracts obtained using two different extraction techniques, namely microwave-assisted and conventional extractions. To optimize the recovery of a large range of metabolites, three extraction times (10, 30 and 60 minutes) and three solvents (methanol, chloroform and acetone) were used. The microwave-assisted extraction gave the highest recovery in the case of methanol and acetone, whereas conventional extraction was the best procedure for chloroform.
Keywords: Azadirachta indica, leaves extract, microwave-assisted extraction, Neem, NMR.
Graphical Abstract
Current Bioactive Compounds
Title:Optimization of the Microwave-Assisted Extraction of Azadirachta indica (Neem) Leaves Using NMR-based Metabolic Fingerprinting
Volume: 11 Issue: 3
Author(s): Simone Carradori, Luisa Mannina, Federica De Cosmi, Tamara Beccarini, Daniela Secci and Anatoly P. Sobolev
Affiliation:
Keywords: Azadirachta indica, leaves extract, microwave-assisted extraction, Neem, NMR.
Abstract: Neem (Azadirachta indica) leaves have been widely proposed as a traditional medicine and many bioactive compounds have been isolated and characterized. Taking into account the high variability of the constituents content, which is influenced by many factors such as the environment and the crop production technology, a proper and fast analytical method is important for monitoring the chemical composition of the product. Here, we report, for the first time, the NMR-based metabolic fingerprinting of Neem leaves extracts obtained using two different extraction techniques, namely microwave-assisted and conventional extractions. To optimize the recovery of a large range of metabolites, three extraction times (10, 30 and 60 minutes) and three solvents (methanol, chloroform and acetone) were used. The microwave-assisted extraction gave the highest recovery in the case of methanol and acetone, whereas conventional extraction was the best procedure for chloroform.
Export Options
About this article
Cite this article as:
Carradori Simone, Mannina Luisa, De Cosmi Federica, Beccarini Tamara, Secci Daniela and P. Sobolev Anatoly, Optimization of the Microwave-Assisted Extraction of Azadirachta indica (Neem) Leaves Using NMR-based Metabolic Fingerprinting, Current Bioactive Compounds 2015; 11 (3) . https://dx.doi.org/10.2174/1573407211666151002001919
DOI https://dx.doi.org/10.2174/1573407211666151002001919 |
Print ISSN 1573-4072 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6646 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Increased OPG Expression and Impaired OPG/TRAIL Ratio in the Aorta of Diabetic Rats
Medicinal Chemistry The Synthesis of Nano-Doxorubicin and its Anticancer Effect
Anti-Cancer Agents in Medicinal Chemistry Chemical Synthesis, Characterisation and Biological Evaluation of Furanic- Estradiol Derivatives as Inhibitors of 17β-Hydroxysteroid Dehydrogenase Type 1
Medicinal Chemistry Recent Advances in Small Molecule Inhibitors of VEGFR and EGFR Signaling Pathways
Current Topics in Medicinal Chemistry Molecular Targets and Angiogenesis in Renal Cell Carcinoma, A Multitarget Approach: Mini Review
Current Drug Targets Metastatic Cell Dormancy and Re-activation: An Overview on Series of Molecular Events Critical for Cancer Relapse
Anti-Cancer Agents in Medicinal Chemistry Kinase Inhibitor Conjugates
Current Pharmaceutical Design Unfolded Protein Response is Involved in Trans-Platinum (II) Complex-Induced Apoptosis in Prostate Cancer Cells via ROS Accumulation
Anti-Cancer Agents in Medicinal Chemistry Smart Polymers for Controlled Delivery of Proteins and Peptides: A Review of Patents
Recent Patents on Drug Delivery & Formulation Nebulizer Therapy in Pulmonology: Review of the Literature
Current Respiratory Medicine Reviews In Vitro and In Vivo Antitumor Efficacy of Docetaxel and Sorafenib Combination in Human Pancreatic Cancer Cells
Current Cancer Drug Targets Editorial: Signalling Pathways in Virus-caused Cancers
Current Cancer Drug Targets Interactions of Iron Oxide Nanoparticles with the Immune System: Challenges and Opportunities for their Use in Nano-oncology
Current Pharmaceutical Design Hyperprolactinemia Secondary to Pituitary Microadenoma Versus Haloperidol- A Diagnostic Enigma. A Case Report and Brief Review
Current Drug Safety Inhibition of Autophagy Strengthens Celastrol-Induced Apoptosis in Human Pancreatic Cancer In Vitro and In Vivo Models
Current Molecular Medicine QSAR Study and Design of Novel Organoselenium and α-Tocopherol Derivatives with Enhanced Chemotherapeutic Activity
Letters in Drug Design & Discovery Peptides As Therapeutics with Enhanced Bioactivity
Current Medicinal Chemistry p53 Regulation of Energy Metabolism and Mitochondria Regulation of p53 in Cancer Cells: An Insight into the Role of Manganese Superoxide Dismutase
Current Pharmaceutical Biotechnology MALDI Mass Spectrometry Imaging, from its Origins up to Today: The State of the Art
Combinatorial Chemistry & High Throughput Screening The Estrogenic Burden on Vascular Risk in Male-to-Female Transsexuals
Current Pharmaceutical Design