A Derivative Method with Free Radical Oxidation to Predict Resveratrol Metabolites by Tandem Mass Spectrometry

Title:A Derivative Method with Free Radical Oxidation to Predict Resveratrol Metabolites by Tandem Mass Spectrometry

Volume: 11 Issue: 4

Author(s): Wangta Liu, Yow-Ling Shiue, Yi-Reng Lin, Hugo You-Hsien Lin and Shih-Shin Liang

Affiliation:

Keywords: Free radical, metabolite, resveratrol, ammonium persulfate (APS), liquid chromatography tandem mass spectrometry (LC–MS/MS), multiple reaction monitoring (MRM).

Abstract: In this study, we demonstrated an oxidative method with free radical to generate 3,5,4-trihydroxy-transstilbene (trans-resveratrol) metabolites and detect sequentially by an autosampler coupling with liquid chromatography electrospray ionization tandem mass spectrometer (LC-ESI–MS/MS). In this oxidative method, the free radical initiator, ammonium persulfate (APS), was placed in a sample bottle containing resveratrol to produce oxidative derivatives, and the reaction progress was tracked by autosampler sequencing. Resveratrol, a natural product with purported cancer preventative qualities, produces metabolites including dihydroresveratrol, 3,4-dihydroxy-trans-stilbene, lunularin, resveratrol monosulfate, and dihydroresveratrol monosulfate by free radical oxidation. Using APS free radical, the concentrations of resveratrol derivatives differ as a function of time. Besides simple, convenient and time- and labor saving, the advantages of free radical oxidative method of its in situ generation of oxidative derivatives followed by LC-ESI–MS/MS can be utilized to evaluate different metabolites in various conditions.

Cite this article as:

Liu Wangta, Shiue Yow-Ling, Lin Yi-Reng, Lin You-Hsien Hugo and Liang Shih-Shin, A Derivative Method with Free Radical Oxidation to Predict Resveratrol Metabolites by Tandem Mass Spectrometry, Current Analytical Chemistry 2015; 11 (4) . https://dx.doi.org/10.2174/1573411011666150515233817