Biophysical Characterisation and Quantification of Nucleic Acid-Protein Interactions: EMSA, MST and SPR

Title:Biophysical Characterisation and Quantification of Nucleic Acid-Protein Interactions: EMSA, MST and SPR

Volume: 16 Issue: 8

Author(s): Janine K. Flores, Ruvini Kariawasam, Adrian X. Gimenez, Stephanie Helder, Liza Cubeddu, Roland Gamsjaeger and Sandro F. Ataide

Affiliation:

Keywords: RNA-Protein interaction, DNA-protein interaction, SRP72, SRP RNA, hSSB2, ssDNA, EMSA, Microscale Thermophoresis, Surface Plasmon Resonance.

Abstract: Cell viability is only possible due to a dynamic range of essential nucleic acid-protein complex formation. DNA replication and repair, gene expression, transcription and protein synthesis are well-known processes mediated by nucleic acids (DNA and RNA) - protein interactions. Novel nucleic acid- protein complexes have been identified in the past few years aided by the development of numerous new techniques such as RNA capture or Tandem RNA Affinity Purification (TRAP). However, the biophysical and biochemical details of these interactions are mostly unknown. Here, we present three techniques (Electrophoretic Mobility Shift Assays, Microscale Thermophoresis and Surface Plasmon Resonance) that are commonly used to quantify and characterize DNA-protein and RNA-protein interactions and discuss their main advantages and limitations.

Cite this article as:

Flores K. Janine, Kariawasam Ruvini, Gimenez X. Adrian, Helder Stephanie, Cubeddu Liza, Gamsjaeger Roland and Ataide F. Sandro, Biophysical Characterisation and Quantification of Nucleic Acid-Protein Interactions: EMSA, MST and SPR, Current Protein & Peptide Science 2015; 16 (8) . https://dx.doi.org/10.2174/1389203716666150505230806