High Cytotoxicity and Apoptotic Effects of Natural Bioactive Benzofuran Derivative on the MCF-7 Breast Cancer Cell Line

Title:High Cytotoxicity and Apoptotic Effects of Natural Bioactive Benzofuran Derivative on the MCF-7 Breast Cancer Cell Line

Volume: 18 Issue: 5

Author(s): Afsane Soleimani, Jahanbakhsh Asadi, Faramarz Rostami-Charati and Roghaye Gharaei

Affiliation:

Keywords: Apoptosis, benzofuran derivative, Cytotoxicity, MCF-7 breast cancer cell line, petasites hybridus.

Abstract: This study was focused on evaluation of the cytotoxicity and apoptotic affects of benzofuran derivative on MCF-7 breast cancer cell line. This effective compound was isolated from the root of Petasites hybridus plant. For experiments, the MCF-7 cells were treated with several concentrations (0-500μM) of 1-(6-hydroxy-2- isopropenyl-1-benzofuran-5-yl)-1-ethanone 1 at different times. In this study, test of neutral red was also employed for cytotoxicity assay and quantity of P53, P21. Bax genes expression was analyzed using Real-Time PCR and ELISA techniques. Results show that compound 1 has cytotoxicity and apoptotic effects on Human breast cancer (Michigan Cancer Foundation-7) MCF-7 cells.

Cite this article as:

Soleimani Afsane, Asadi Jahanbakhsh, Rostami-Charati Faramarz and Gharaei Roghaye, High Cytotoxicity and Apoptotic Effects of Natural Bioactive Benzofuran Derivative on the MCF-7 Breast Cancer Cell Line, Combinatorial Chemistry & High Throughput Screening 2015; 18 (5) . https://dx.doi.org/10.2174/1386207318666150430114815