CPP-mediated Protein Delivery in a Noncovalent Form: Proof-of-Concept for Percutaneous and Intranasal Delivery

Title:CPP-mediated Protein Delivery in a Noncovalent Form: Proof-of-Concept for Percutaneous and Intranasal Delivery

Volume: 21 Issue: 11

Author(s): Zhao Wang, Yingzhi Chen, Ergang Liu, Junbo Gong, Meong Cheol Shin and Yongzhuo Huang

Affiliation:

Keywords: Cell-penetrating peptide, noncovalent, nose-to-brain delivery, percutaneous delivery, protein delivery, TAT.

Abstract: Macromolecular drugs (e.g., proteins and nucleic acids) are highly environmentally liable and unstable, and their administration is strictly limited to injection. Moreover, a vast majority of macromolecules are cell membrane- impermeable, and it is a critical issue to enhance the cellular uptake efficiency for improving the treatment outcomes. Cell-penetrating peptide (CPP)-assisted strategy is promising for effective macromolecular delivery. As a case in point, CPP-mediated protein delivery has been considered as a revolutionary breakthrough. With aid of CPP, virtually all pro- teins can become cell-permeable. Generally, CPP-protein delivery works in a covalent delivery pattern, by which CPP and its cargo are linked via covalent bond. Recently, noncovalent delivery has also attracted attention for its potential application for protein delivery. In the presented work, the noncovalent pattern was demonstrated for its feasibi lity in percutaneous and nose-to-brain delivery with TAT/GFP as model drug, in comparison with the covalent method. Noncovalent CPP/protein delivery and its noninvasive application may provide a facile method for protein therapy.

Cite this article as:

Wang Zhao, Chen Yingzhi, Liu Ergang, Gong Junbo, Shin Cheol Meong and Huang Yongzhuo, CPP-mediated Protein Delivery in a Noncovalent Form: Proof-of-Concept for Percutaneous and Intranasal Delivery , Protein & Peptide Letters 2014; 21 (11) . https://dx.doi.org/10.2174/0929866521666140807121903