Transfection Optimization of Hyperbranched Poly 2-Dimethylaminoethyl Methacrylate (pDMAEMA) based Polymers In Vitro

Title:Transfection Optimization of Hyperbranched Poly 2-Dimethylaminoethyl Methacrylate (pDMAEMA) based Polymers In Vitro

Volume: 2 Issue: 1

Author(s): Asha Mathew, Wenxin Wang and Abhay Pandit

Affiliation:

Keywords: Cellular metabolism, PEGylation, poly (2-dimethyl-aminoethylmethacrylate) (pDMAEMA), protonation, transfection.

Abstract: The present study reports the transfection and cytotoxicity of hyperbranched poly (2-dimethylaminoethylmethacrylate) (pDMAEMA) based polymer at various physiological conditions. Transfection capability and cytotoxicity of the hyperbranched polymer were first evaluated by varying the molecular weight upon PEGylation. Hyperbranched polymer with optimal PEGylation was later protonized and the effect of protonation was evaluated. Finally, the effects of serum on transfection and cellular metabolic activity on various cell types were investigated. Results suggest that hyperbranched polymer with medium PEGylation (Mw 24000Da) and protonation were best suited for transfection which exhibited minimal toxicity. However, the effect of serum was cell-type dependent.

Cite this article as:

Mathew Asha, Wang Wenxin and Pandit Abhay, Transfection Optimization of Hyperbranched Poly 2-Dimethylaminoethyl Methacrylate (pDMAEMA) based Polymers In Vitro, Pharmaceutical Nanotechnology 2014; 2 (1) . https://dx.doi.org/10.2174/2211738501666140110231305