Inhibition of Candida rugosa Lipase by Secondary Metabolites Extracts of Three Algerian Plants and their Antioxydant Activities

K.      Benarous; A.      Djeridane; A.      Kameli; M.      Yousfi

doi:10.2174/1573408011309010010

Abstract

Lipase inhibitors have generated a great interest because they could help in the prevention or the therapy of lipase‐related diseases. Therefore, the aim of this work was to evaluate the inhibitory effect of secondary metabolites extracts such as phenolic compounds and saponins of three Algerian medicinal plants: Achillea santolina, Inonotus hispidus and Zizyphus lotus, indeed their antiradicalaire activity using DPPH• (1, 1‐diphenyl‐2‐picryl‐hydrazyl). The phenolic extracts have shown a strong antiradicalaire activity than the saponin extracts with EC50 values ranged from 6 to 11 μg/ml and from 51 to 82 μg/ml, respectively. The enzymatic inhibition produced by these plant extracts is described here for the first time. The results have shown that the phenolic extracts are more potent than the saponin extracts with Ki values ranged from 0.011 mg/ml to 0.027 mg/ml for phenolic extracts, and ranged from 0.071 mg/ml to 0.69 mg/ml for saponin extracts. The nature, mechanism and possible physiological relevance of lipase inhibition by extract components are discussed.

Keywords: Achillea santolina, Inonotus hispidus, Zizyphus lotus, Candida rugosa lipase, inhibition, saponins extracts, phenolic extracts, DPPH•.