Toxins from Physalia physalis (Cnidaria) Raise the Intracellular Ca2+ of Beta-Cells and Promote Insulin Secretion

Title:Toxins from Physalia physalis (Cnidaria) Raise the Intracellular Ca2+ of Beta-Cells and Promote Insulin Secretion

Volume: 19 Issue: 31

Author(s): C.M. Diaz-Garcia, D. Fuentes-Silva, C. Sanchez-Soto, D. Dominguez-Perez, N. Garcia-Delgado, C. Varela, G. Mendoza-Hernandez, A. Rodriguez-Romero, O. Castaneda and M. Hiriart

Affiliation:

Keywords: Animal experiment, cnidarian venom, cytosolic calcium, glucose tolerance test, insulin release, ion channel, islet beta-cell, mass spectrometry, Physalia physalis, toxins

Abstract: Physalia physalis is a marine cnidarian from which high molecular weight toxins with hemolytic and neurotoxic effects have been isolated. In the present work, two novel toxins, PpV9.4 and PpV19.3 were purified from P. physalis by bioactive guideline isolation. It involved two steps of column chromatography, gel filtration and RP-HPLC. The molecular weights were 550.7 and 4720.9 Da for PpV9.4 and PpV19.3, respectively. In the light of the Edman sequencing results, the structure of these toxins included the presence of modified amino acids. Both toxins increased the percentage of insulin secreting beta-cells and induced cytosolic Ca2+ elevation. To date, this is the first report of low molecular weight toxins increasing insulin secretion purified from cnidarians, by constituting a new approach to the study of beta-cells physiology.

Cite this article as:

Diaz-Garcia C.M., Fuentes-Silva D., Sanchez-Soto C., Dominguez-Perez D., Garcia-Delgado N., Varela C., Mendoza-Hernandez G., Rodriguez-Romero A., Castaneda O. and Hiriart M., Toxins from Physalia physalis (Cnidaria) Raise the Intracellular Ca2+ of Beta-Cells and Promote Insulin Secretion, Current Medicinal Chemistry 2012; 19 (31) . https://dx.doi.org/10.2174/092986712803833308