In Vitro Comparative Evaluation of Non-Leaves and Leaves Extracts of Andrographis paniculata on Modulation of Inflammatory Mediators

C.      V. Chandrasekaran; B.      Murali; M.      Deepak; Amit      Agarwal

doi:10.2174/187152312803305731

Abstract

This study was designed to evaluate and compare the inhibitory property of extracts of Andrographis paniculata leaves [methanolic (AP1), hydroalcoholic (AP2), successive water (AP3)] and non-leaves [methanolic (AP4), hydroalcoholic (AP5), successive water (AP6)] towards inflammatory mediators (NO, IL-1 beta, IL-6, TNF alpha, PGE2, TXB2 and LTB4). Stimulant induced J774A.1 murine macrophages and HL-60 promyelocytic leukemic cells were used to study the inhibitory potential of extracts of A. paniculata on inflammatory mediators. Results revealed that AP1 and AP4 exhibited inhibitory effect on all the inflammatory mediators excluding PGE2 and TNF-alpha. AP2 and AP5 exhibited inhibitory effect towards IL-1 beta, TXB2 and did not show inhibitory effect towards other mediators. However, AP3 and AP6 failed to show inhibitory activity against any of the inflammatory mediators at the tested concentrations. Further, we observed that the magnitude of inhibitory effect displayed by A. paniculata extracts depends on the andrographolide content, although, it does not appear to influence the inhibitory effect towards LTB4 production.

Keywords: Andrographis paniculata, leaves, non-leaves, inflammatory mediators (NO, IL-1 beta, IL-6, LTB4, PGE2, TNF-alpha and TXB2)