Controversies About the Chromosomal Stability of Cultivated Mesenchymal Stem Cells: Their Clinical Use is it Safe?

Title:Controversies About the Chromosomal Stability of Cultivated Mesenchymal Stem Cells: Their Clinical Use is it Safe?

Volume: 7 Issue: 5

Author(s): Reginaldo Justino Ferreira, Ana Carolina Irioda, Ricardo Correa Cunha, Julio Cesar Francisco, Luiz Cesar Guarita-Souza, Garikipati Venkata Naga Srikanth, Soniya Nityanand, Roberto Rosati, Juan Carlos Chachques and Katherine Athayde Teixeira de Carvalho

Affiliation:

Keywords: Adult stem cell, chromosomal abnormalities, FISH, genetic screening, in vitro transformation, karyotyping, MSC cultivation, neoplastic transformation of MSC, stem cell (SC) biology.

Abstract: The usefulness of adult stem cells in research and therapeutic applications highly relies on their genomic integrity and stability. Many laboratories including ours have addressed this concern using methods such as karyotyping, Qbanding, fluorescent in situ hybridization, array CGH, flow cytometry and Pap test to evaluate number and structure of chromosomes and cellular phenotype. This review attempts to summarize the findings reported so far for the studies on chromosomal aberrations in adult stem cells and warrant to perform certain basic tests before transplantation to avoid any adverse reactions, which will thus aid in better therapeutic output after cellular transplantation in the treatment of various diseases.

Cite this article as:

Justino Ferreira Reginaldo, Carolina Irioda Ana, Correa Cunha Ricardo, Cesar Francisco Julio, Cesar Guarita-Souza Luiz, Venkata Naga Srikanth Garikipati, Nityanand Soniya, Rosati Roberto, Carlos Chachques Juan and Athayde Teixeira de Carvalho Katherine, Controversies About the Chromosomal Stability of Cultivated Mesenchymal Stem Cells: Their Clinical Use is it Safe?, Current Stem Cell Research & Therapy 2012; 7 (5) . https://dx.doi.org/10.2174/157488812802481472