D-Serine-Dehydratase from Saccaromyces cerevisiae: A Pyridoxal 5’- phosphate-Dependent Enzyme for Advanced Biotech Applications

Maria      Staiano; Maria      Strianese; Antonio      Varriale; Stefano      Di Giovanni; Donatella      Scotto di Mase; Valentina      Dell’Angelo; Giuseppe      Ruggiero; Tullio      Labella; Claudio      Pellecchia; Sabato      D’Auria

doi:10.2174/092986612800494002

Abstract

The Saccaromices cerevisiae D-serine dehydratase is a pyridoxal 5'-phosphate dependent enzyme that requires zinc for its function. It catalyses the conversion of D-serine into pyruvate and ammonia with the K(m) and k(cat) values of 0.39 mM and 13.1 s(-1) respectively. In this work, a new methodology for monitoring D-serine is presented. Our results show that this enzyme could be successfully used as a biological probe for detection of D-serine via fluorescence spectroscopy.

Keywords: Fluorescence, biosensors, D-Serine dehydratase, absorption, pyridoxal 5'-phosphate, fluorescence spectroscopy, NMDA (N-methyl-D-aspartate), D-serine, neuro-diseases, enzymatic assays

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Protein & Peptide Letters

Title:D-Serine-Dehydratase from Saccaromyces cerevisiae: A Pyridoxal 5’- phosphate-Dependent Enzyme for Advanced Biotech Applications

Volume: 19 Issue: 6

Author(s): Maria Staiano, Maria Strianese, Antonio Varriale, Stefano Di Giovanni, Donatella Scotto di Mase, Valentina Dell’Angelo, Giuseppe Ruggiero, Tullio Labella, Claudio Pellecchia and Sabato D’Auria

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Keywords: Fluorescence, biosensors, D-Serine dehydratase, absorption, pyridoxal 5'-phosphate, fluorescence spectroscopy, NMDA (N-methyl-D-aspartate), D-serine, neuro-diseases, enzymatic assays

Abstract: The Saccaromices cerevisiae D-serine dehydratase is a pyridoxal 5'-phosphate dependent enzyme that requires zinc for its function. It catalyses the conversion of D-serine into pyruvate and ammonia with the K(m) and k(cat) values of 0.39 mM and 13.1 s(-1) respectively. In this work, a new methodology for monitoring D-serine is presented. Our results show that this enzyme could be successfully used as a biological probe for detection of D-serine via fluorescence spectroscopy.

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Staiano Maria, Strianese Maria, Varriale Antonio, Di Giovanni Stefano, Scotto di Mase Donatella, Dell’Angelo Valentina, Ruggiero Giuseppe, Labella Tullio, Pellecchia Claudio and D’Auria Sabato, D-Serine-Dehydratase from Saccaromyces cerevisiae: A Pyridoxal 5’- phosphate-Dependent Enzyme for Advanced Biotech Applications, Protein & Peptide Letters 2012; 19 (6) . https://dx.doi.org/10.2174/092986612800494002