A Luminescence Study of the Interaction of Sulfobutylether-β-Cyclodextrin with Rutin

Title: A Luminescence Study of the Interaction of Sulfobutylether-β-Cyclodextrin with Rutin

Volume: 5 Issue: 4

Author(s): Min Wu, Zhenghua Song and Jingjing Zhang

Affiliation:

Keywords: Chemiluminescence, flow injection, luminal, rutin, pharmacokinetics, sulfobutylether-β-cyclodextrin, Cyclodextrins, oligosaccharides, bioavailability, photochemical reaction

Abstract: The luminol/sulfobutylether-β-cyclodextrin (SBE7M-β-CD) chemiluminescence (CL) system and the interaction of SBE7M-β-CD/rutin were first described by flow injection (FI) CL method. It was found that SBE7M-β-CD with luminol could form 1:1 complex online, which could accelerate the electrons transferring rate of excited 3-aminophthalate, giving the enhanced CL intensity of luminol. The enhancement of CL intensity was proportional to the concentrations of SBE7M- β-CD with a linear range from 25 to 1750 μmol L-1. It was also found that rutin could inhibit the CL intensity from luminol/SBE7M-β-CD system, and the decrement of CL intensity was logarithm over the concentrations of rutin ranging from 0.1 to 100.0 nmol L-1, giving the regression equation ΔI = 32.90lgCrutin + 16.26 (R2 = 0.9952) with a detection limit of 0.03 nmol L-1 (3σ). According to the proposed CL model, the binding constant (KCD-R) and the stoichiometric ratio of SBE7M-β-CD/rutin complex were obtained as 1.6 × 106 L2 mol-2 and 2:1. The possible mechanism of luminol/SBE7M-β- CD/rutin interaction was also discussed. The method was successfully applied to monitor rutin in human urine samples after ingesting SBE7M-β-CD/rutin complex, with a total excretion of 68.8% within 8.0 h.

Cite this article as:

Wu Min, Song Zhenghua and Zhang Jingjing, A Luminescence Study of the Interaction of Sulfobutylether-β-Cyclodextrin with Rutin, Drug Metabolism Letters 2011; 5 (4) . https://dx.doi.org/10.2174/187231211798472520