Furin Cleavage of Bacterial Expressed Glutathione-S-Transferase-Pro-Transforming Growth Factor β1 Fusion Protein In Vitro

N.   A.   Kahle; C.      Joffroy; S.   L.   Popp; C.      Knabbe; M.   B.   Stope

doi:10.2174/092986610790963609

Abstract

To investigate the processing of transforming growth factor β1 (TGFβ1) pro-protein by furin protease we expressed a GST-pro-TGFβ1 fusion protein in bacteria. Analysis of the furin digestion pattern revealed the liberation of 12.5 kDa TGFβ1 monomers. There was no evidence for cleavage of an alternative furin site within the pro-protein.

Keywords: Transforming growth factor β1, furin, proteolytic processing

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Protein & Peptide Letters

Title: Furin Cleavage of Bacterial Expressed Glutathione-S-Transferase-Pro-Transforming Growth Factor β1 Fusion Protein In Vitro

Volume: 17 Issue: 4

Author(s): N. A. Kahle, C. Joffroy, S. L. Popp, C. Knabbe and M. B. Stope

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Keywords: Transforming growth factor β1, furin, proteolytic processing

Abstract: To investigate the processing of transforming growth factor β1 (TGFβ1) pro-protein by furin protease we expressed a GST-pro-TGFβ1 fusion protein in bacteria. Analysis of the furin digestion pattern revealed the liberation of 12.5 kDa TGFβ1 monomers. There was no evidence for cleavage of an alternative furin site within the pro-protein.

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Kahle A. N., Joffroy C., Popp L. S., Knabbe C. and Stope B. M., Furin Cleavage of Bacterial Expressed Glutathione-S-Transferase-Pro-Transforming Growth Factor β1 Fusion Protein In Vitro, Protein & Peptide Letters 2010; 17 (4) . https://dx.doi.org/10.2174/092986610790963609