Stationary Phase Expression, Purification, and Characterization of XorKI, a Restriction Endonuclease from Xanthomonas oryzae pv. oryzae

Woo   Young   Kang; Young   Kee   Chae

doi:10.2174/092986610790780378

Abstract

An endonuclease from Xanthomonas oryzae pathovar oryzae KACC 10331, XorKI, was heterologously produced in Escherichia coli by applying the stationary state induction method. The yield was 5.4 mg of XorKI per liter of LB medium. XorKI existed in multiple oligomeric forms as evidenced by gel filtration chromatography. The specific activity of purified XorKI was 323000 units per mg.

Keywords: XorKI, restriction endonuclease, Xanthomonas, stationary state induction

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Protein & Peptide Letters

Title: Stationary Phase Expression, Purification, and Characterization of XorKI, a Restriction Endonuclease from Xanthomonas oryzae pv. oryzae

Volume: 17 Issue: 3

Author(s): Woo Young Kang and Young Kee Chae

Affiliation:

Keywords: XorKI, restriction endonuclease, Xanthomonas, stationary state induction

Abstract: An endonuclease from Xanthomonas oryzae pathovar oryzae KACC 10331, XorKI, was heterologously produced in Escherichia coli by applying the stationary state induction method. The yield was 5.4 mg of XorKI per liter of LB medium. XorKI existed in multiple oligomeric forms as evidenced by gel filtration chromatography. The specific activity of purified XorKI was 323000 units per mg.

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Kang Young Woo and Chae Kee Young, Stationary Phase Expression, Purification, and Characterization of XorKI, a Restriction Endonuclease from Xanthomonas oryzae pv. oryzae, Protein & Peptide Letters 2010; 17 (3) . https://dx.doi.org/10.2174/092986610790780378