Characterization of a New Defensin from Cowpea (Vigna unguiculata (L.) Walp.)

Lara      Padovan; Ludovica      Segat; Alessandro      Tossi; Tercilio      Calsa Jr.; Akio   Kido   Ederson; Lucas      Brandao; Rafael   L.   Guimaraes; Valesca      Pandolfi; Maria   Clara   Pestana-Calsa; Luis   Carlos   Belarmino; Ana   Maria   Benko-Iseppon; Sergio      Crovella

doi:10.2174/092986610790780350

Abstract

Using Phaseoleae defensins available in databases, a putative defensin gene was isolated in cowpea (Vigna unguiculata (L.) Walp.) and cloned from genomic cowpea DNA. The putative mature defensin sequence displays the characteristic defensins residues arrangement, secondary and tertiary structures were predicted and splicing analysis was performed. Using RT-PCR, defensin expression and differences in response to biotic stimuli between infected and non infected plants were tested.

Keywords: Antimicrobial peptides, cowpea mosaic virus, evolution, plant defensin, protein function, Vigna unguiculata

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Protein & Peptide Letters

Title: Characterization of a New Defensin from Cowpea (Vigna unguiculata (L.) Walp.)

Volume: 17 Issue: 3

Author(s): Lara Padovan, Ludovica Segat, Alessandro Tossi, Tercilio Calsa Jr., Akio Kido Ederson, Lucas Brandao, Rafael L. Guimaraes, Valesca Pandolfi, Maria Clara Pestana-Calsa, Luis Carlos Belarmino, Ana Maria Benko-Iseppon and Sergio Crovella

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Keywords: Antimicrobial peptides, cowpea mosaic virus, evolution, plant defensin, protein function, Vigna unguiculata

Abstract: Using Phaseoleae defensins available in databases, a putative defensin gene was isolated in cowpea (Vigna unguiculata (L.) Walp.) and cloned from genomic cowpea DNA. The putative mature defensin sequence displays the characteristic defensins residues arrangement, secondary and tertiary structures were predicted and splicing analysis was performed. Using RT-PCR, defensin expression and differences in response to biotic stimuli between infected and non infected plants were tested.

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Padovan Lara, Segat Ludovica, Tossi Alessandro, Calsa Jr. Tercilio, Ederson Kido Akio, Brandao Lucas, Guimaraes L. Rafael, Pandolfi Valesca, Pestana-Calsa Clara Maria, Belarmino Carlos Luis, Benko-Iseppon Maria Ana and Crovella Sergio, Characterization of a New Defensin from Cowpea (Vigna unguiculata (L.) Walp.), Protein & Peptide Letters 2010; 17 (3) . https://dx.doi.org/10.2174/092986610790780350