FCS-Based Sensing for the Detection of Ochratoxin and Neomycin in Food

Title: FCS-Based Sensing for the Detection of Ochratoxin and Neomycin in Food

Volume: 16 Issue: 12

Author(s): Antonio Varriale, Maria Staiano, Luisa Iozzino, Lorella Severino, Aniello Anastasio, Maria Luisa Cortesi and Sabato D'Auria

Affiliation:

Keywords: Food safety, Micotoxins, FCS, Biosensors, Antibodies

Abstract: In this work, we present an advanced fluorescence assay for the detection of traces of ocratoxin A and neomycin in food. The described assay is based on measurement of the fluctuations of the fluorescein-labeled analytes by a focused laser beam in the absence and in the presence of the specific antibodies anti-analytes. A competitive assay based on the utilization of unlabeled analytes was developed. The obtained results indicated that the combination of high-avidity IgG antibodies together with an innovative fluorescence immunoassay strategy resulted in the detection limit of 0.0078 ng and 0.0156 ng for ochratoxin A and neomycin, respectively.

Cite this article as:

Varriale Antonio, Staiano Maria, Iozzino Luisa, Severino Lorella, Anastasio Aniello, Cortesi Luisa Maria and D'Auria Sabato, FCS-Based Sensing for the Detection of Ochratoxin and Neomycin in Food, Protein & Peptide Letters 2009; 16 (12) . https://dx.doi.org/10.2174/092986609789839359