Roles of the Valine Clusters in Domain 3 of the Hemolytic Lectin CEL-III in Its Oligomerization and Hemolytic Abilities

Keigo      Hisamatsu; Hideaki      Unno; Shuichiro      Goda; Tomomitsu      Hatakeyama

doi:10.2174/092986609787848054

Abstract

The hemolytic lectin CEL-III and its site-directed mutants were expressed in Escherichia coli cells. Replacement of the valine clusters in domain 3 with alanine residues led to increased self-oligomerization in solution and higher hemolytic activity. The results suggest the involvement of these valine clusters in CEL-III oligomerization and hemolytic activity.

Keywords: Cucumaria echinata, hemolysin, lectin, oligomerization, site-directed mutagenesis

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Protein & Peptide Letters

Title: Roles of the Valine Clusters in Domain 3 of the Hemolytic Lectin CEL-III in Its Oligomerization and Hemolytic Abilities

Volume: 16 Issue: 4

Author(s): Keigo Hisamatsu, Hideaki Unno, Shuichiro Goda and Tomomitsu Hatakeyama

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Keywords: Cucumaria echinata, hemolysin, lectin, oligomerization, site-directed mutagenesis

Abstract: The hemolytic lectin CEL-III and its site-directed mutants were expressed in Escherichia coli cells. Replacement of the valine clusters in domain 3 with alanine residues led to increased self-oligomerization in solution and higher hemolytic activity. The results suggest the involvement of these valine clusters in CEL-III oligomerization and hemolytic activity.

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Hisamatsu Keigo, Unno Hideaki, Goda Shuichiro and Hatakeyama Tomomitsu, Roles of the Valine Clusters in Domain 3 of the Hemolytic Lectin CEL-III in Its Oligomerization and Hemolytic Abilities, Protein & Peptide Letters 2009; 16 (4) . https://dx.doi.org/10.2174/092986609787848054