Screening of SMG7-Binding Peptides by Combination of Phage Display and Docking Simulation Analysis

Title: Screening of SMG7-Binding Peptides by Combination of Phage Display and Docking Simulation Analysis

Volume: 16 Issue: 3

Author(s): Muhammed Zahed, Toshikazu Suzuki, Akiko Suganami, Hajime Sugiyama, Kazuo Harada, Masaki Takiguchi, Yutaka Tamura and Nobuo Suzuki

Affiliation:

Keywords: Phage display, in silico screening, docking simulation, pull-down assay

Abstract: We screened SMG7-binding peptides with phage display and docking simulation analysis. Although a consensus motif was absent in the phage display-derived candidates, we succeeded to find a peptide CDDRPPKSC, which can bind specifically to SMG7. We conclude that docking simulation helps to find high-affinity peptides efficiently, even if the phage display-screened candidates lack a consensus region.

Cite this article as:

Zahed Muhammed, Suzuki Toshikazu, Suganami Akiko, Sugiyama Hajime, Harada Kazuo, Takiguchi Masaki, Tamura Yutaka and Suzuki Nobuo, Screening of SMG7-Binding Peptides by Combination of Phage Display and Docking Simulation Analysis, Protein & Peptide Letters 2009; 16 (3) . https://dx.doi.org/10.2174/092986609787601741