Two-dimensional Protein Analysis by Isoelectric Focusing in a Multi-cell Plate System

Title: Two-dimensional Protein Analysis by Isoelectric Focusing in a Multi-cell Plate System

Volume: 9 Issue: 1

Author(s): Beatriz Dos Santos Ferreira, Alan Trindade Branco, Karlla Salim de Queiroz and Goncalo Apolinario de Souza Filho

Affiliation:

Keywords: isoelectric protein focusing (IEF), sodium dodecylsulfate (SDS)-PAGE, mercaptoethanol, Ampholytes solution, maize endosperm

Abstract: A practical and low cost system for isoelectric protein focusing (IEF) was developed. The system uses a multi-cell glass plate compatible with a common vertical one-dimensional electrophoresis chamber, dispensing specific IEF apparatus. After focusing, the IEF gels are easily recovered. The resulting two-dimensional (2-D) gels have provided efficient protein separation for different concentrations and extracts.

Cite this article as:

Ferreira Dos Santos Beatriz, Branco Trindade Alan, de Queiroz Salim Karlla and de Souza Filho Apolinario Goncalo, Two-dimensional Protein Analysis by Isoelectric Focusing in a Multi-cell Plate System, Protein & Peptide Letters 2002; 9 (1) . https://dx.doi.org/10.2174/0929866023409057