Crystallization and Preliminary Crystallographic Analysis of Laminarinase from Rhodothermus marinus: A Case of Pseudomerohedral Twinning

Title: Crystallization and Preliminary Crystallographic Analysis of Laminarinase from Rhodothermus marinus: A Case of Pseudomerohedral Twinning

Volume: 15 Issue: 10

Author(s): Alexander M. Golubev, Adriana L. Rojas, Alessandro S. Nascimento, Lucas Bleicher, Anna A. Kulminskaya, Elena V. Eneyskaya and Igor Polikarpov

Affiliation:

Keywords: Laminarinase, Rhodothermus marinus, crystallization, pseudomerohedral twinning

Abstract: Thermophilic endo-1,3(4)-β-glucanase (laminarinase) from Rhodothermus marinus was crystallized by the hanging-drop vapor diffusion method. The needle-like crystals belong to space group P21 and contain two protein molecules in the asymmetric unit with a solvent content of 51.75 %. Diffraction data were collected to a resolution of 1.95Å and resulted in a dataset with an overall Rmerge of 10.4% and a completeness of 97.8%. Analysis of the structure factors revealed pseudomerohedral twinning of the crystals with a twin fraction of approximately 42%.

Cite this article as:

Golubev M. Alexander, Rojas L. Adriana, Nascimento S. Alessandro, Bleicher Lucas, Kulminskaya A. Anna, Eneyskaya V. Elena and Polikarpov Igor, Crystallization and Preliminary Crystallographic Analysis of Laminarinase from Rhodothermus marinus: A Case of Pseudomerohedral Twinning, Protein & Peptide Letters 2008; 15 (10) . https://dx.doi.org/10.2174/092986608786071139