Construction, Expression, Purification and Immunology Effect of an Antiatherosclerosis Chimeric Enzyme Vaccine in Escherichia coli

Title: Construction, Expression, Purification and Immunology Effect of an Antiatherosclerosis Chimeric Enzyme Vaccine in Escherichia coli

Volume: 15 Issue: 7

Author(s): Sun Yunxiao, Li Zhufang, Yang Xin, Long Jun, Xiong Qiyan, Qi Gaofu, Cao Rongyue, Wu Jie, Liu Jingjing, Fan Hao and Li Taiming

Affiliation:

Keywords: Atherosclerosis, vaccine, CETP B cell epitope, antibody, construction, expression and purification

Abstract: In order to prevent atherosclerosis, a chimeric enzyme vaccine of AnsB-TTP-PADRE-CETPC was successfully constructed, expressed and purified to immunize New Zealand white rabbits for inducing high titers of anti-CETP antibodies to improve lipid abnormality. The protein was expressed as soluble protein in Escherichia coli and purified by anion exchange column and Sephadex G-100 size-exclusion chromatography. After immunizing rabbits with the purified protein, high titer anti-CETP antibodies were induced and lasted more than nineteen weeks in vivo; High density lipoprotein cholesterol (HDL-C) content in the serum was elevated to 61% while decreased low density lipoprotein cholesterol (LDL-C) to 37.2% compared with control rabbits in the presence of Al(OH)3.

Cite this article as:

Yunxiao Sun, Zhufang Li, Xin Yang, Jun Long, Qiyan Xiong, Gaofu Qi, Rongyue Cao, Jie Wu, Jingjing Liu, Hao Fan and Taiming Li, Construction, Expression, Purification and Immunology Effect of an Antiatherosclerosis Chimeric Enzyme Vaccine in Escherichia coli, Protein & Peptide Letters 2008; 15 (7) . https://dx.doi.org/10.2174/092986608785133654