Propionate Sensor Using Coenzyme-A Transferase and Acyl-CoA Oxidase

Koji      Sode; Wakako      Tsugawa; Minoru      Aoyagi; Eranna      Rajashekhara; Kazuya      Watanabe

doi:10.2174/092986608785203737

Abstract

We developed an amperometric propionate sensor using comprised of two recombinant enzymes, propionate coenzyme A CoA transferase from Clostridium propionicum and short-chain acyl-CoA oxidase from Arabidopsis thaliana. Response current increased linearly with increase in propionate concentration from 10 μM to 100 μM. The detection limit was 10 μM propionate.

Keywords: Propionate, volatile fatty acid, propionate CoA transferase, short-chain acyl-CoA oxidase, amperometry, enzyme sensor

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Protein & Peptide Letters

Title: Propionate Sensor Using Coenzyme-A Transferase and Acyl-CoA Oxidase

Volume: 15 Issue: 8

Author(s): Koji Sode, Wakako Tsugawa, Minoru Aoyagi, Eranna Rajashekhara and Kazuya Watanabe

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Keywords: Propionate, volatile fatty acid, propionate CoA transferase, short-chain acyl-CoA oxidase, amperometry, enzyme sensor

Abstract: We developed an amperometric propionate sensor using comprised of two recombinant enzymes, propionate coenzyme A CoA transferase from Clostridium propionicum and short-chain acyl-CoA oxidase from Arabidopsis thaliana. Response current increased linearly with increase in propionate concentration from 10 μM to 100 μM. The detection limit was 10 μM propionate.

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Sode Koji, Tsugawa Wakako, Aoyagi Minoru, Rajashekhara Eranna and Watanabe Kazuya, Propionate Sensor Using Coenzyme-A Transferase and Acyl-CoA Oxidase, Protein & Peptide Letters 2008; 15 (8) . https://dx.doi.org/10.2174/092986608785203737