Characterization of Fluoroalcohols-Induced Intermediates of Mucor miehei Lipase at Low pH

Sadaf      Fatima; Aseem      Mishra; Priyankar      Sen; Rizwan   Hasan   Khan

doi:10.2174/092986608784246425

Abstract

We have previously characterized an acid-unfolded (UA) state of Mucor miehei lipase at pH 2. The effect of 2,2,2-trifluoroethanol (TFE) and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) resulted in characterization of molten-globule (MG) like states with β-sheet secondary structure at 15% (v/v) TFE and 6% (v/v) HFIP. α-Helical states accumulate at 80% (v/v) TFE and 30% (v/v) HFIP.

Keywords: Lipase, Mucor miehei, Molten Globule like state, circular dichroism, partially folded intermediate

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Protein & Peptide Letters

Title: Characterization of Fluoroalcohols-Induced Intermediates of Mucor miehei Lipase at Low pH

Volume: 15 Issue: 4

Author(s): Sadaf Fatima, Aseem Mishra, Priyankar Sen and Rizwan Hasan Khan

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Keywords: Lipase, Mucor miehei, Molten Globule like state, circular dichroism, partially folded intermediate

Abstract: We have previously characterized an acid-unfolded (UA) state of Mucor miehei lipase at pH 2. The effect of 2,2,2-trifluoroethanol (TFE) and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) resulted in characterization of molten-globule (MG) like states with β-sheet secondary structure at 15% (v/v) TFE and 6% (v/v) HFIP. α-Helical states accumulate at 80% (v/v) TFE and 30% (v/v) HFIP.

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Fatima Sadaf, Mishra Aseem, Sen Priyankar and Khan Hasan Rizwan, Characterization of Fluoroalcohols-Induced Intermediates of Mucor miehei Lipase at Low pH, Protein & Peptide Letters 2008; 15 (4) . https://dx.doi.org/10.2174/092986608784246425