Abstract
A precursor of ConBr, a glucose/mannose-binding plant lectin, was expressed in the yeast Pichia pastoris. Western blot analysis of transformed cells detected an intracellularly recombinant protein band with ca. 34.5 kDa. The recombinant protein was apparently active as suggested by its strong interaction with the mannose-rich yeast cell debris.
Keywords: Recombinant protein, carbohydrate-binding, phytohemagglutinin, yeast
Protein & Peptide Letters
Title: Expression of a Canavalia brasiliensis Lectin (ConBr) Precursor in Pichia pastoris
Volume: 15 Issue: 4
Author(s): C. P.S. Carvalho, C. S. Rocha, D. R. Nepomuceno, J. T.A. Oliveira and T. B. Grangeiro
Affiliation:
Keywords: Recombinant protein, carbohydrate-binding, phytohemagglutinin, yeast
Abstract: A precursor of ConBr, a glucose/mannose-binding plant lectin, was expressed in the yeast Pichia pastoris. Western blot analysis of transformed cells detected an intracellularly recombinant protein band with ca. 34.5 kDa. The recombinant protein was apparently active as suggested by its strong interaction with the mannose-rich yeast cell debris.
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Cite this article as:
Carvalho P.S. C., Rocha S. C., Nepomuceno R. D., Oliveira T.A. J. and Grangeiro B. T., Expression of a Canavalia brasiliensis Lectin (ConBr) Precursor in Pichia pastoris, Protein & Peptide Letters 2008; 15 (4) . https://dx.doi.org/10.2174/092986608784246579
DOI https://dx.doi.org/10.2174/092986608784246579 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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