Crystallization and Preliminary X-Ray Studies of TON_0559, a Putative Member of the Haloacid Dehalogenase (HAD) Superfamily from Thermococcus onnurineus NA1

Title: Crystallization and Preliminary X-Ray Studies of TON_0559, a Putative Member of the Haloacid Dehalogenase (HAD) Superfamily from Thermococcus onnurineus NA1

Volume: 15 Issue: 2

Author(s): Kyeong Kyu Kim, Chi My Thi Nguyen, Hyun Sook Lee, Yona Cho, Jung-Hyun Lee, Sung Chul Ha, Hye-Yeon Hwang, Chi My Thi Nguyen, Hyun Sook Lee, Sung Chul Ha and Kyeong Kyu Kim

Affiliation:

Keywords: TON_0559, crystallization, Thermococcus onnurineus

Abstract: To elucidate the molecular basis underlying their broad substrate specificity and reaction mechanism of the enzymes belonging to the haloacid dehalogenase (HAD) superfamily, TON_0559, a putative HAD subfamily protein from a hyperthermophilic archaeon Thermococcus onnurineus NA1, was expressed, purified and crystallized. X-ray diffraction data were collected to 2.0 Å resolution. The space group is C2, with unit cell parameters a = 121.2 Å, b = 62.9 Å, c = 37.5 Å and β= 106.5°.

Cite this article as:

Kim Kyu Kyeong, Thi Nguyen My Chi, Lee Sook Hyun, Cho Yona, Lee Jung-Hyun, Ha Chul Sung, Hwang Hye-Yeon, My Thi Nguyen Chi, Sook Lee Hyun, Chul Ha Sung and Kyu Kim Kyeong, Crystallization and Preliminary X-Ray Studies of TON_0559, a Putative Member of the Haloacid Dehalogenase (HAD) Superfamily from Thermococcus onnurineus NA1, Protein & Peptide Letters 2008; 15 (2) . https://dx.doi.org/10.2174/092986608783489625