Sonication Induced Intermediate in Prion Protein Conversion

John   Mark   Carter; Audrius   A.   Zukas; Cathrin   E.   Bruederle; Audrius      A. Zukas; Cathrin      E. Bruederle; John      Mark Carter

doi:10.2174/092986608783489517

Abstract

We have observed that hamster prion protein (PrPC) undergoes conformational changes on exposure to heat or sonication. If a sonication induced new conformer is seeded with a small amount of its abnormal pathogenic isoform (PrPSc) it undergoes a significant conversion to a proteinase-resistant isoform. This suggests the presence of a third stable PrP conformer, which may be intermediate in the conversion of PrPC to PrPSc.

Keywords: Prion protein, scrapie, PMCA, sonication, protein aggregation

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Protein & Peptide Letters

Title: Sonication Induced Intermediate in Prion Protein Conversion

Volume: 15 Issue: 2

Author(s): John Mark Carter, Audrius A. Zukas, Cathrin E. Bruederle, Audrius A. Zukas, Cathrin E. Bruederle and John Mark Carter

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Keywords: Prion protein, scrapie, PMCA, sonication, protein aggregation

Abstract: We have observed that hamster prion protein (PrPC) undergoes conformational changes on exposure to heat or sonication. If a sonication induced new conformer is seeded with a small amount of its abnormal pathogenic isoform (PrPSc) it undergoes a significant conversion to a proteinase-resistant isoform. This suggests the presence of a third stable PrP conformer, which may be intermediate in the conversion of PrPC to PrPSc.

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Carter Mark John, Zukas A. Audrius, Bruederle E. Cathrin, A. Zukas Audrius, E. Bruederle Cathrin and Mark Carter John, Sonication Induced Intermediate in Prion Protein Conversion, Protein & Peptide Letters 2008; 15 (2) . https://dx.doi.org/10.2174/092986608783489517