Crystallization and X-Ray Analysis of Rhodothermus marinus Cytochrome c at 1.23 Å Resolution

Meike      Stelter; Ana   M.P.   Melo; Ligia   M.   Saraiva; Miguel      Teixeira; Margarida      Archer

doi:10.2174/092986607782541024

Abstract

Cytochrome c from Rhodothermus marinus has been crystallized using the hanging-drop vapor-diffusion method in 30 % (w/v) polyethylene glycol 8K, 0.2 M ammonium sulfate, 8 % hexanediol and 50 mM sodium citrate pH 2.2. The crystals belong to space group P21. X-ray diffraction data were collected to 1.23 Å resolution using synchrotron radiation and a wavelength of 0.93 Å.

Keywords: Cytochrome c, thermophiles, Rhodothermus marinus, crystallization

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Protein & Peptide Letters

Title: Crystallization and X-Ray Analysis of Rhodothermus marinus Cytochrome c at 1.23 Å Resolution

Volume: 14 Issue: 10

Author(s): Meike Stelter, Ana M.P. Melo, Ligia M. Saraiva, Miguel Teixeira and Margarida Archer

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Keywords: Cytochrome c, thermophiles, Rhodothermus marinus, crystallization

Abstract: Cytochrome c from Rhodothermus marinus has been crystallized using the hanging-drop vapor-diffusion method in 30 % (w/v) polyethylene glycol 8K, 0.2 M ammonium sulfate, 8 % hexanediol and 50 mM sodium citrate pH 2.2. The crystals belong to space group P21. X-ray diffraction data were collected to 1.23 Å resolution using synchrotron radiation and a wavelength of 0.93 Å.

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Stelter Meike, Melo M.P. Ana, Saraiva M. Ligia, Teixeira Miguel and Archer Margarida, Crystallization and X-Ray Analysis of Rhodothermus marinus Cytochrome c at 1.23 Å Resolution, Protein & Peptide Letters 2007; 14 (10) . https://dx.doi.org/10.2174/092986607782541024