Isolation and Partial Characterization of A β-Glucuronidase of the Mollusk Pomacea sp.

Maria   Lucia   Lira de Andrade; Vanessa   Duarte   de Morais; Joao   Felipe   de Sousa Filho

doi:10.2174/092986607782541097

Abstract

This paper studies the β-glucuronidase in the mollusk Pomacea sp. The β-glucuronidase was isolated 206-fold with a 1,5% yield and the cinetc parameters was: pH 5.0, 65°C, Km of 72 x 10-2 mM and molecular mass of 116 kDa. HPLC confirmed the purity. BaCl2 increased β-glucuronidase activity and SDS and NaH2PO4 inhibited completely.

Keywords: β- glucuronidase, glycosaminoglycans, high-performance liquid, chromatography (HPLC), Pomacea sp

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Protein & Peptide Letters

Title: Isolation and Partial Characterization of A β-Glucuronidase of the Mollusk Pomacea sp.

Volume: 14 Issue: 10

Author(s): Maria Lucia Lira de Andrade, Vanessa Duarte de Morais and Joao Felipe de Sousa Filho

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Keywords: β- glucuronidase, glycosaminoglycans, high-performance liquid, chromatography (HPLC), Pomacea sp

Abstract: This paper studies the β-glucuronidase in the mollusk Pomacea sp. The β-glucuronidase was isolated 206-fold with a 1,5% yield and the cinetc parameters was: pH 5.0, 65°C, Km of 72 x 10-2 mM and molecular mass of 116 kDa. HPLC confirmed the purity. BaCl2 increased β-glucuronidase activity and SDS and NaH2PO4 inhibited completely.

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Lira de Andrade Lucia Maria, de Morais Duarte Vanessa and de Sousa Filho Felipe Joao, Isolation and Partial Characterization of A β-Glucuronidase of the Mollusk Pomacea sp., Protein & Peptide Letters 2007; 14 (10) . https://dx.doi.org/10.2174/092986607782541097