Crystallizationand Preliminary X-Ray Crystallographic Studies of Formaldehyde Dehydrogenase from Pseudomonas putida

Title: Crystallizationand Preliminary X-Ray Crystallographic Studies of Formaldehyde Dehydrogenase from Pseudomonas putida

Volume: 8 Issue: 3

Author(s): Yoshio Kusakabe, Nobuatada Tanaka, Kiyoshi Ito, Tadashi Yoshimoto and Kazuo T. Nakamura

Affiliation:

Keywords: Formaldehyde Dehydrogenase, Pseudomonas Putida, PFDH molecule, glutathione-dependent FDHs

Abstract: The formaldehyde dehydrogenease from Pseudomonas putida (PFDH), has been crystallized by the vapour diffusion method using ammonium sulfate as the Precipitating agent. The crystals belong to a trigonal space group P3 12 (or P3 12) with the cell dimensions of a = b = 85.74 A, and c = 190.9 A. There are two subunits per asymmetric unit. The crystals diffract to at least 2.2 A resolution using Cu K Alfa radiation at 100 K. Self-rotation function studies suggest that the tetrameric PFDH molecular has the 222 point group symmetry.

Cite this article as:

Kusakabe Yoshio, Tanaka Nobuatada, Ito Kiyoshi, Yoshimoto Tadashi and Nakamura T. Kazuo, Crystallizationand Preliminary X-Ray Crystallographic Studies of Formaldehyde Dehydrogenase from Pseudomonas putida, Protein & Peptide Letters 2001; 8 (3) . https://dx.doi.org/10.2174/0929866013409454