Functional Expression of a Galactose-Binding C-Type Lectin In Pichia pastoris

Jia-da      Li; Qiang      Xu; Ke-yi      Wang

doi:10.2174/0929866013409463

Abstract

Trimeresurus stejnegeri lectin (TSL) was expressed in the yeast Pichia pastoris under the control of alcohol oxidase (AOX1) promoter. The recombinant protein, which was glycosylated, can be purified by one-step affinity chromatography. The purified recombinant TSL shared similar sugar-binding activity and Specificity with the native protein as determined by hemagglutination and enzyme-linked lectin binding Assays.

Keywords: C-TYPE LECTIN, PICHIA PASTORIS, Trimeresurus stejnegeri lectin (TSL), alcohol oxidase (AOX1) promoter, galactose-binding C-type, mutagenesis, alcohol oxidase (AOX1)

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Protein & Peptide Letters

Title: Functional Expression of a Galactose-Binding C-Type Lectin In Pichia pastoris

Volume: 8 Issue: 3

Author(s): Jia-da Li, Qiang Xu and Ke-yi Wang

Affiliation:

Keywords: C-TYPE LECTIN, PICHIA PASTORIS, Trimeresurus stejnegeri lectin (TSL), alcohol oxidase (AOX1) promoter, galactose-binding C-type, mutagenesis, alcohol oxidase (AOX1)

Abstract: Trimeresurus stejnegeri lectin (TSL) was expressed in the yeast Pichia pastoris under the control of alcohol oxidase (AOX1) promoter. The recombinant protein, which was glycosylated, can be purified by one-step affinity chromatography. The purified recombinant TSL shared similar sugar-binding activity and Specificity with the native protein as determined by hemagglutination and enzyme-linked lectin binding Assays.

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Li Jia-da, Xu Qiang and Wang Ke-yi, Functional Expression of a Galactose-Binding C-Type Lectin In Pichia pastoris, Protein & Peptide Letters 2001; 8 (3) . https://dx.doi.org/10.2174/0929866013409463