Isolation and Identification of Two Membrane Proteins Binding to Transfer RNA

Quan-Sheng      Liu; Jian-Hua      Liu; De-Sheng      Jiang; You-Xin      Jin; De-Bao      Wang

doi:10.2174/0929866013409265

Abstract

Two major tRNA binding proteins were obtained by biochemical method from yeast crude extracts of total membrane proteins. They were further confirmed to be tRNA specific-binding proteins by gel mobility shift assay. N-terminal sequence assays showed that they were glyceraldehyde-3-phosphate dehydranase (GAPDH) and 3-phosphate glycerate kinase (PGK1), respectively.

Keywords: glyceraldehyde-3-phosphate dehydranase (GAPDH), RNA polymerase III

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Protein & Peptide Letters

Title: Isolation and Identification of Two Membrane Proteins Binding to Transfer RNA

Volume: 8 Issue: 5

Author(s): Quan-Sheng Liu, Jian-Hua Liu, De-Sheng Jiang, You-Xin Jin and De-Bao Wang

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Keywords: glyceraldehyde-3-phosphate dehydranase (GAPDH), RNA polymerase III

Abstract: Two major tRNA binding proteins were obtained by biochemical method from yeast crude extracts of total membrane proteins. They were further confirmed to be tRNA specific-binding proteins by gel mobility shift assay. N-terminal sequence assays showed that they were glyceraldehyde-3-phosphate dehydranase (GAPDH) and 3-phosphate glycerate kinase (PGK1), respectively.

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Liu Quan-Sheng, Liu Jian-Hua, Jiang De-Sheng, Jin You-Xin and Wang De-Bao, Isolation and Identification of Two Membrane Proteins Binding to Transfer RNA, Protein & Peptide Letters 2001; 8 (5) . https://dx.doi.org/10.2174/0929866013409265