Affinity of Chicken Cystatin for S- (Carbamoylmethyl)-Papain, Measured by Fluorescence at Acidic ph

Alfonso      Arroyo-Reyna; Ezequiel      Gutierrez-Gomez

doi:10.2174/0929866023408797

Abstract

We have characterized the interaction between S-(carbamoylmethyl)-papain and chicken cystatin by means of fluorescence titration at acidic pH. The calculated binding constant was 32 x 10 6, which is less than the tight affinity at pH 7.4 (∼7 x 10 10). Results have been discussed in terms of the burial of Trp-104 of cystatin and the possible role of the charge of the carbamoylmethyl moiety.

Keywords: carbamoylmethyl-papain, chicken cystatin

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Protein & Peptide Letters

Title: Affinity of Chicken Cystatin for S- (Carbamoylmethyl)-Papain, Measured by Fluorescence at Acidic ph

Volume: 9 Issue: 3

Author(s): Alfonso Arroyo-Reyna and Ezequiel Gutierrez-Gomez

Affiliation:

Keywords: carbamoylmethyl-papain, chicken cystatin

Abstract: We have characterized the interaction between S-(carbamoylmethyl)-papain and chicken cystatin by means of fluorescence titration at acidic pH. The calculated binding constant was 32 x 10 6, which is less than the tight affinity at pH 7.4 (∼7 x 10 10). Results have been discussed in terms of the burial of Trp-104 of cystatin and the possible role of the charge of the carbamoylmethyl moiety.

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Arroyo-Reyna Alfonso and Gutierrez-Gomez Ezequiel, Affinity of Chicken Cystatin for S- (Carbamoylmethyl)-Papain, Measured by Fluorescence at Acidic ph, Protein & Peptide Letters 2002; 9 (3) . https://dx.doi.org/10.2174/0929866023408797