Biophysical Evidence of Lipid and Carbohydrate Binding Activities of Shrimp High Density Lipoprotein / B Glucan Binding Protein

Title: Biophysical Evidence of Lipid and Carbohydrate Binding Activities of Shrimp High Density Lipoprotein / B Glucan Binding Protein

Volume: 9 Issue: 4

Author(s): Claudia L. Vargas-Requena, Alejandra Hernandez-Santoyo, Gloria Yepiz-Plascencia, Francisco Vargas-Albores, Inocencio Higuera-Ciapara, Adela Rodriguez-Romero, Jose L. Soulages and Rogerio R. Sotelo-Mundo

Affiliation:

Keywords: High Density Lipoprotei, Glucan Binding Protein, heparin, laminaribiose

Abstract: Crustacean High Density Lipoprotein / β-Glucan Binding Protein (HDL / BGBP) has been studied due to its role in nutrition and immune response via activation of the defense cells (hemocytes) upon binding 1,3-D-β-glucan carbohydrates. In this study, HDL / BGBP was found to be composed mainly of beta sheets, as determined by circular dichroism. Lipoprotein aggregation resulted when HDL / BGBP interacted with phospolipid vesicles, laminaribiose (1,3-β-glucan disaccharide) or heparin. HDL / BGBP has similar dissociation constants for laminaribiose (Kd=22 mM) or heparin (Kd=46 mM) as determined by 90° light scattering.

Cite this article as:

Vargas-Requena L. Claudia, Hernandez-Santoyo Alejandra, Yepiz-Plascencia Gloria, Vargas-Albores Francisco, Higuera-Ciapara Inocencio, Rodriguez-Romero Adela, Soulages L. Jose and Sotelo-Mundo R. Rogerio, Biophysical Evidence of Lipid and Carbohydrate Binding Activities of Shrimp High Density Lipoprotein / B Glucan Binding Protein, Protein & Peptide Letters 2002; 9 (4) . https://dx.doi.org/10.2174/0929866023408706