Functional Expression of Human Methionine Aminopeptidase Type 1 in Saccharomyces Cerevisiae

Title: Functional Expression of Human Methionine Aminopeptidase Type 1 in Saccharomyces Cerevisiae

Volume: 9 Issue: 4

Author(s): Benjamin Dummitt, Ying Fei and Yie-Hwa Chang

Affiliation:

Keywords: Human Methionine, Aminopeptidase, Saccharomyces Cerevisiae, anti-fungal drugs

Abstract: We expressed recombinant human methionine aminopeptidase type 1 (MAP or MetAP) in a map1 null yeast strain to determine the extent of functional complementation between the two proteins. The human MetAP1 protein fully rescued the slow growth phenotype associated with deletion of yeast MetAP1, suggesting that the yeast and human MetAP1 proteins may have similar roles in vivo. Expression of human MetAP1 in yeast has significance in understanding the function of the human protein, studying its in vivo substrate specificity, and developing specific anti-fungal drugs to target yeast MetAP1.

Cite this article as:

Dummitt Benjamin, Fei Ying and Chang Yie-Hwa, Functional Expression of Human Methionine Aminopeptidase Type 1 in Saccharomyces Cerevisiae, Protein & Peptide Letters 2002; 9 (4) . https://dx.doi.org/10.2174/0929866023408607